JCRB0150 P53LMACO1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0150 Cell Name   P53LMACO1
Profile   Smooth muscle cell line established from thoracic aorta of a p53 knock out mouse. Other Name   
Animal   mouse Strain   outbred line of 129Sv & C57BL6
Genus   Mus Species   musclus
Sex    Age   3-month-old
Identity    Tissue for Primary Cancer   vascular system
Case history   p53 knock out mouse Metastasis   No
Tissue Metastasized    Genetics   p53 deficient(-/-)
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   smooth muscle cell line from p53 knock out mouse aorta
Classify   normal Established by   Ohmi,K. et.al.
Registered by   Ohmi,K. Regulation for Distribution   free
Comment    Year   1999
Medium   Dulbecco's modified Eagle's medium with high glucose and fetal bovine serum. Low glucose will reduce an expression of the alpha smooth muscle actin. Methods for Passages   Cells harvested after treatment with 0.25% trypsin and 0.02% EDTA. Subculture every 3-4 days.
Cell Number on Passage   2.5 x 10^4 cells/sq.cm. Race   
CO2 Conc.   5 % Tissue Sampling   thoracic aorta
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_U220
Reference
Pubmed id:9299470A novel aortic smooth muscle cell line obtained from p53 knock out mice expresses several differentiation characteristics.
Ohmi K,Masuda T,Yamaguchi H,Sakurai T,Kudo Y,Katsuki M,Nonomura Y
Biochem Biophys Res Commun. 1997 Sep 8;238(1):154-8
Pubmed id:8048955Gene replacement of the p53 gene with the lacZ gene in mouse embryonic stem cells and mice by using two steps of homologous recombination.
Gondo Y,Nakamura K,Nakao K,Sasaoka T,Ito K,Kimura M,Katsuki M
Biochem Biophys Res Commun. 1994 Jul 29;202(2):830-7

Images
Movies



LOT Information

Cell No.   JCRB0150 Cell Name   
LOT No.   09232004 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (high glucose, final 4.5 g/L) with 10% fetal bovine serum (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   4 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. Subculture every 3-4 days.
Doubling Time   ca. 1 day Cell Number in Vial (cells/1ml)   2.2 x 10^6
Viability at cell freezing (%)   96.7 Antibiotics Used   free
Passage Number   P9* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0150 Cell Name   P53LMACO1
LOT No.   06212000 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with high glucose (4.5g/L) and 10% fetal calf serum (Intergen RB2405). Temperature   37 C
Cell Density at Seeding   1.1 x 10^5 cells/sq.cm. Methods for Passages   Cells harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.8 x 10^6
Viability at cell freezing (%)   90.7 Antibiotics Used   free
Passage Number   P6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD, NP, LDH. Mouse.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion    Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0150 Cell Name   P53LMACO1
LOT No.   08252014 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (high glucose modification, 4.5 g/L) with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091). Usual low glucose DMEM will reduce an expression of the alpha smooth muscle actin. Temperature   37 C
Cell Density at Seeding   5.9 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   96 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM (high glucose) CO2 Conc.   5%
Viability immediately after thawing (%)   96 Additional information   
Cell No.   JCRB0150 Cell Name   P53LMACO1
LOT No.   10122017 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (high glucose) and fetal bovine serum (Sigma Cat. # 17J2012). Low glucose will reduce an expression of the alpha smooth muscle actin. Temperature   37 C
Cell Density at Seeding   3.3 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 21 hrs. Cell Number in Vial (cells/1ml)   3.9 x 10^6
Viability at cell freezing (%)   85 Antibiotics Used   free
Passage Number   Unknown (9 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM (high glucose) CO2 Conc.   5%
Viability immediately after thawing (%)   85 Additional information   
CLOSE