JCRB0143.3 F2408 EBNA2 cl-18

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0143.3 Cell Name   F2408 EBNA2 cl-18
Profile   Rat embryo cell line transfected by recombinant plasmid pZE2 with coding region of the EBNA2. Other Name   F2408 EBNA2
Animal   rat Strain   Fischer
Genus   Rattus Species   norvegicus
Sex    Age   embryo
Identity    Tissue for Primary Cancer   embryo
Case history   recombinant plasmid pZE2 with coding region of the EBNA2 was transfected Metastasis   
Tissue Metastasized    Genetics   Expression of the EBNA2 is strong.
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   fibroblast-like
Classify   transformed Established by   W. Eckhalt
Registered by   Shimakage,M. Regulation for Distribution   free
Comment    Year   1999
Medium   Dulbecco's modified Eagle's medium with 5% fetal calf serum. Methods for Passages   Cells are treated with 0.25% trypsin and 0.02% EDTA when subculture cells and subculture cells every 4 days.
Cell Number on Passage   2 x 10^5 cells/6 cm dish Race   
CO2 Conc.   5 % Tissue Sampling   whole body of embryo
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_E945
Reference
Pubmed id:8938143Increased sensitivity of EBNA2-transformed rat fibroblasts to ionizing radiation.
Shimakage M,Miyata Y,Inoue H,Yutsudo M,Hakura A
Int J Cancer. 1996 Nov 27;68(5):612-5
Pubmed id:7556601Tumorigenicity of EBNA2-transfected cells.
Shimakage M,Kurata A,Inoue H,Okamoto Y,Yutsudo M,Hakura A
FEBS Lett. 1995 Sep 11;371(3):245-8
Pubmed id:6307528Cellular responsiveness to growth factors correlates with a cell's ability to express the transformed phenotype.
Kaplan PL,Ozanne B
Cell. 1983 Jul;33(3):931-8

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LOT Information

Cell No.   JCRB0143.3 Cell Name   F2408 EBNA2 cl-18
LOT No.   09182000 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 5% fetal calf serum (Mitsubishi PFCS PVF001). Temperature   37 C
Cell Density at Seeding   0.5 x 10^4 cells/sq.cm. Methods for Passages   Cells harvested after treatment with 0.25% trypsin and 0.02% EDTA. Change medium one day after starting culture. Subculture every 5 dyas.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.6 x 10^6
Viability at cell freezing (%)   98.3 Antibiotics Used   free
Passage Number   P5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP,LDH,G6PD. Rat. by Sasaki,A.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium containing 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0143.3 Cell Name   F2408 EBNA2 cl-18
LOT No.   08102000 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 5% fetal calf serum (Mitsubishi, PFCS PVF001). Temperature   37 C
Cell Density at Seeding   2.0 x 10^4 cells/sq.cm. Methods for Passages   Cells harvested after treatment with 0.25% trypsin and 0.02% EDTA. Medium change every 2-3 days, subculture every 3-4 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.9 x 10^6
Viability at cell freezing (%)   98.6 Antibiotics Used   free
Passage Number   P5* PDL   NT
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   +
Sterility: FUNGI   NT Isozyme Analysis   G6PD, NP, LDH. Rat. (Sasaki,A.).
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium containing 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
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