JCRB0158.2 MY-M13

Cell information

Important Notice(s)

Media for JCRB0157 TK, JCRB0158.0-0158.2 MY**, JCRB0159 SLVL

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0158.2 Cell Name   MY-M13
Profile   Subclone of the MY cells, human bone marrow mononulcear cells. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   52
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   patient diagnosed as having acute biphenotypic leukemia. See reference for details. Metastasis   
Tissue Metastasized    Genetics   Chimeric BCR/ABL, BCR exon e1 and ABL exon a3. der(9)t(9;22), t(2;3)(p16,q29).
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   Bone marrow mononuclear cells.
Classify   tumor Established by   Inokuchi,K. et. al.
Registered by   Inokuchi,K. Regulation for Distribution   not defined
Comment    Year   2000
Medium   RPMI1640 medium with 10% fetal calf serum Methods for Passages   dilution
Cell Number on Passage   split Race   Japanese
CO2 Conc.   5 % Tissue Sampling   peripheral blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3036
Reference
Pubmed id:9790503Establishment of a cell line with variant BCR/ABL breakpoint expressing P180BCR/ABL from late-appearing Philadelphia-positive acute biphenotypic leukemia.
Inokuchi K,Shinohara T,Futaki M,Hanawa H,Tanosaki S,Yamaguchi H,Nomura T,Dan K
Genes Chromosomes Cancer. 1998 Nov;23(3):227-38

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LOT Information

Cell No.   JCRB0158.2 Cell Name   MY-M13
LOT No.   03072001 Lot Specification   distribution
Medium   alpha-MEM medium with 10% heat inactivated fetal calf serum (Intergen RB2405). Temperature   37 C
Cell Density at Seeding   P5* Methods for Passages   Simple dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.3 x 10^6
Viability at cell freezing (%)   96.1 Antibiotics Used   free
Passage Number   P5* PDL   NT
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   G6PD(typeB), NP, LDH examined. human non HeLa.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,13
D13S317:8
D7S820:10,13
D16S539:9
VWA:17,18
TH01:9
AM:X
TPOX:8,11
CSF1PO:11,12
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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