JCRB0158.0 MY

Cell information

Important Notice(s)

Media for JCRB0157 TK, JCRB0158.0-0158.2 MY**, JCRB0159 SLVL

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0158.0 Cell Name   MY
Profile   Human bone marrow mononuclear cells derived from a patient having acute biphenotypic leukemia. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   52
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   patient diagnosed as having acute biphenotypic leukemia. See reference for details. Metastasis   
Tissue Metastasized    Genetics   Chimeric BCR/ABL, BCR exon e1 and ABL exon a3. der(9)t(9;22), t(2;3)(p16;q29).
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   Bone marrow mononuclear cells.
Classify   tumor Established by   Inokuchi,K. et. al.
Registered by   Inokuchi,K. Regulation for Distribution   not defined
Comment    Year   2000
Medium   RPMI1640 medium with 10% fetal calf serum Methods for Passages   dilution
Cell Number on Passage   split Race   Japanese
CO2 Conc.   5 % Tissue Sampling   peripheral blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3034
Reference
Pubmed id:9790503Establishment of a cell line with variant BCR/ABL breakpoint expressing P180BCR/ABL from late-appearing Philadelphia-positive acute biphenotypic leukemia.
Inokuchi K,Shinohara T,Futaki M,Hanawa H,Tanosaki S,Yamaguchi H,Nomura T,Dan K
Genes Chromosomes Cancer. 1998 Nov;23(3):227-38

Images



LOT Information

Cell No.   JCRB0158.0 Cell Name   MY
LOT No.   05172001 Lot Specification   distribution
Medium   alpha-MEM medium with 10% heat inactivated fetal calf serum (Intergen RB2405). Temperature   37 C
Cell Density at Seeding   5 x 10^5 cells/ml Methods for Passages   Cells are subcultured by dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.3 x 10^6
Viability at cell freezing (%)   95.0 Antibiotics Used   free
Passage Number   P5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, LDH and G6PD(G6PD) examined. Human not HeLa.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,13
D13S317:8
D7S820:10,13
D16S539:9
VWA:17,18
TH01:9
AM:X
TPOX:8,11
CSF1PO:11,12
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Culture medium with 5 % DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0158.0 Cell Name   MY
LOT No.   05182001 Lot Specification   distribution
Medium   alpha-MEM with 10% heat-inactivated fetal calf serum (Intergen RB2405). Temperature   37 C
Cell Density at Seeding   5 x 10^5 cells/ml Methods for Passages   Simple dilution. Suspension cell culture.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   92.3 Antibiotics Used   free
Passage Number   P5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB), LDH and NP examined. Human not HeLa.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Culture medium containing 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0158.0 Cell Name   MY
LOT No.   03292001 Lot Specification   distribution
Medium   Alpha-MEM medium with 10% heat inactivated fetal calf serum (Intergen RB2405). Temperature   37 C
Cell Density at Seeding   1.0 x 10^5 cells/ml Methods for Passages   Simple dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)   91.6 Antibiotics Used   free
Passage Number   P5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Sample is beeing preparing again.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
CLOSE