JCRB0153.3 tsJT60
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0153.3 | Cell Name | tsJT60 |
|---|---|---|---|
| Profile | Rat 3Y1 derived ts mutant cell line with cell cycle arrest at G0 phase. | Other Name | tsJT60(3Y1) |
| Animal | rat | Strain | Fischer 344 |
| Genus | Rattus | Species | norvegicus |
| Sex | F | Age | fetus |
| Identity | Tissue for Primary Cancer | embryo | |
| Case history | normal | Metastasis | |
| Tissue Metastasized | Genetics | A ts mutant for cell cycle progression from G0 to S phase. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | 3Y1 derived ts mutant cell line with MNNG treatment. Cells grow at 34 C and are arrested at G0 at 40 C. Cells continue proliferation at both temperatures in cell cycle. |
| Classify | mutant | Established by | Ninomiya-Tsuji,J. and Ide,T. |
| Registered by | Kyoizumi,S. | Regulation for Distribution | free |
| Comment | Year | 2000 | |
| Medium | Dulbecco's modified Eagle's medium with 0.1% glucose and 10% fetal calf serum. | Methods for Passages | Cells harvested after treatment with 0.125% trypsin and 0.01% EDTA. Subculture once a week at split ratio=1/8 at 34 C. |
| Cell Number on Passage | 5-8 x 10^5 cells/60 mm dish | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | embryo |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:7698232 | Induction of gadd153 gene in G0-specific ts mutant cells at nonpermissive temperature. Kashima N,Ueohzono T,Fornace AJ Jr,Ide T Exp Cell Res. 1995 Apr;217(2):324-8 |
| Pubmed id:2150790 | Nonlethal G0-ts mutant tsJT60 becomes lethal at the nonpermissive temperature after transformation: a hint for new cancer chemotherapeutics. Tai YY,Ninomiya-Tsuji J,Furuoku K,Ogawa N,Ishibashi S,Shiroki K,Segawa K,Tsuchida N,Shibuya M,Ide T Cell Struct Funct. 1990 Dec;15(6):385-91 |
| Pubmed id:3169150 | A cell cycle G0-ts mutant, tsJT60, becomes lethal at the nonpermissive temperature after transformation with adenovirus 12 E1B 19K mutant. Tai YY,Goto Y,Ninomiya-Tsuji J,Kameoka Y,Ishibashi S,Shiroki K,Ide T Exp Cell Res. 1988 Nov;179(1):50-7 |
| Pubmed id:2822238 | Entrance of SV40-transformed cells into G0 phase as revealed by a study using the G0-specific ts mutant tsJT60. Ninomiya-Tsuji J,Ishibashi S,Ide T Cancer Res. 1987 Nov 15;47(22):6028-32 |
| Pubmed id:3499990 | Bypass of the ts block of tsJT60, a G0-specific ts mutant from rat fibroblasts, by fetal bovine serum and epidermal growth factor. Ninomiya-Tsuji J,Nakahara Y,Ito C,Akiyama T,Ishibashi S,Ide T Cell Struct Funct. 1987 Oct;12(5):421-32 |
| Pubmed id:3040451 | Induction of cellular DNA synthesis in G0-specific ts mutant, tsJT60, following infection with SV40 and adenoviruses. Ninomiya-Tsuji J,Goto Y,Ishibashi S,Shiroki K,Ide T Exp Cell Res. 1987 Aug;171(2):509-12 |
| Pubmed id:3497814 | Epidermal growth factor has a unique effect in combination with fetal bovine serum to bypass the ts-block of G0-specific ts mutant tsJT60. Ninomiya-Tsuji J,Nakahara Y,Ito C,Akiyama T,Ishibashi S,Ide T Exp Cell Res. 1987 Jul;171(1):86-93 |
| Pubmed id:3801408 | Expression of growth-regulated genes in tsJT60 cells, a temperature-sensitive mutant of the cell cycle. Ide T,Ninomiya-Tsuji J,Ferrari S,Philiponis V,Baserga R Biochemistry. 1986 Nov 4;25(22):7041-6 |
| Pubmed id:2423350 | Defect in prereplicative phase of G0-specific ts mutant, tsJT60. Ninomiya-Tsuji J,Goto Y,Ishibashi S,Ide T Exp Cell Res. 1986 Jul;165(1):191-8 |
| Pubmed id:6692850 | Isolation of a G0-specific ts mutant from a Fischer rat cell line, 3Y1. Ide T,Ninomiya J,Ishibashi S Exp Cell Res. 1984 Jan;150(1):60-7 |
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LOT Information
| Cell No. | JCRB0153.3 | Cell Name | tsJT60 |
|---|---|---|---|
| LOT No. | 02152001 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with 10% fetal calf serum (Mitsubishi PFCS PVF001). | Temperature | 37 C |
| Cell Density at Seeding | 7.5 x 10^3 cells/sq.cm. | Methods for Passages | Cells are harvested after treatment with 0.125% trypsin and 0.01% EDTA. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.7 x 10^6 |
| Viability at cell freezing (%) | 90.6 | Antibiotics Used | free |
| Passage Number | P10* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | G6PD, NP, LDH examined. (rat). |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Culture medium with 5% DMSO. | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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