-Detailed Information [JCRB0160]-

JCRB0160 LI90

Cell information

Important Notice(s)

Notice for the cultivation of the LI90 cells (JCRB0160) / LI90の培養上の注意

Notice for the cultivation of the LI90 cells (JCRB0160)
JCRB0160 LI90細胞の培養上の注意
--------------------------------------------------------------------------------
Tomokazu Matsu-ura, MD.
Jikei University
May, 29 2000
--------------------------------------------------------------------------------
The LI90 is not immortalized cell line and displays limited life span. It was
reported that the growth of this cell line arrests around 25 passages after
establishment. The passage number of lot is described on data sheet.

1. Culture medium

Regularly Dulbecco's modified Eagle's medium with 10% fetal bovine serum or Williams' E medium with 10% fetal bovine serum are used. In some case ASF104 medium (culture medium for serum free cell culture produced by Ajino-Moto Co.) with 1-5 % fetal bovine serum can be used (see references of the cell line).

2. Methods of subculture

Cells are treated with 0.25% trypsin and 0.05% EDTA at 37 C for 1-2 minutes resulting to detach cells. Cells are then harvested and washed by centrifugation at 1,500rpm for 5 minutes. Split ratio at subculture is between 1:3 and 1:4.

3. Culture dishes

Regular culture dishes are good enough to attach cells and type I collagen coated dishes will cause better cell attachment.

4. Caution for handling cells

Growth rate is approximately 60 hours according to Dr. Murakami who established the cell line. However, growth rate comes to slow down after continuous passages for long period. Thus it is recommended that the cells should be propagated as soon as received and make enough number of ampoules and stored in the liquid nitrogen freezer before starting any experiments. Some one recommended that the increased concentration of serum(20 %) is effective when growth rate becomes slow down.

5. Freezing methods

FM-1 freezing medium reduced a viability for this cells. Thus the FM-1 medium is not recommended in this case. Regular freezing method is recommended for this cell line (DMEM with 10% heat inactivated fetal bovine serum containing 10% DMSO).

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Cell type：general cells (View Pricing Information)

JCRB No.	JCRB0160	Cell Name	LI90
Profile	Ito (fat-storing) cells, stellate-shaped mesenchymal cells that exist in the space of Disse of the liver and contain many fat droplets in cytoplasm.	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex	F	Age	55
Identity	available	Tissue for Primary Cancer	liver, gallbladder
Case history	epithelioid hemangioendothelioma.	Metastasis
Tissue Metastasized		Genetics	Karyology: 47XX, 6q-, +22
Life Span	finite	Crisis PDL
Morphology	epithelial-like	Character
Classify	tumor	Established by	Murakami,K. and etc.
Registered by	Matsuura,T.	Regulation for Distribution	free
Comment		Year	2000
Medium	Dulbecco's modified Eagle's medium with 10% heat-inactivated fetal bovine serum.	Methods for Passages	Cells harvested after treatment with PBS containing 0.25% trypsin and 1 mM EDTA. Change medium twice a week.
Cell Number on Passage	Not specified.	Race	Japanese
CO2 Conc.	5 %	Tissue Sampling	liver
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_0390

Reference
Pubmed id:12811248	Establishment of immortalized human hepatic stellate scavenger cells to develop bioartificial livers. Watanabe T,Shibata N,Westerman KA,Okitsu T,Allain JE,Sakaguchi M,Totsugawa T,Maruyama M,Matsumura T,Noguchi H,Yamamoto S,Hikida M,Ohmori A,Reth M,Weber A,Tanaka N,Leboulch P,Kobayashi N Transplantation. 2003 Jun 15;75(11):1873-80
Pubmed id:10462172	Retinol esterification activity contributes to retinol transport in stellate cells. Matsuura T,Hasumura S,Nagamori S,Murakami K Cell Struct Funct. 1999 Jun;24(3):111-6
Pubmed id:7540235	Establishment of a new human cell line, LI90, exhibiting characteristics of hepatic Ito (fat-storing) cells. Murakami K,Abe T,Miyazawa M,Yamaguchi M,Masuda T,Matsuura T,Nagamori S,Takeuchi K,Abe K,Kyogoku M Lab Invest. 1995 Jun;72(6):731-9

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LOT Information

06082012
11072011
06172011
03042011
09212010
07262010
03152010
11122009
03022009
12192008
09192008
04022008
06252007
04112007
11022006
11302004
12092004
02032004
11062003
10032001
10162001
09142001
09032001
08162001
08172001
06062001
04172001
08212013
09082017
02282020
05222023

Cell No.	JCRB0160	Cell Name
LOT No.	06082012	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10099)	Temperature	37 C
Cell Density at Seeding	2.6 - 5.5 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 2 days	Cell Number in Vial (cells/1ml)	2.8 x 10^5
Viability at cell freezing (%)	93.9	Antibiotics Used	free
Passage Number	P16	PDL	+18.4
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0160	Cell Name
LOT No.	11072011	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium (1 g/L glucose) with 10% fetal bovine serum (FBS, GIBCO Cat. # 10099)	Temperature	37 C
Cell Density at Seeding	2 - 5 x 10^4 cells/ml	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 3-4 days	Cell Number in Vial (cells/1ml)	2.6 x 10^5
Viability at cell freezing (%)	68.4	Antibiotics Used	free
Passage Number	P17	PDL	+21.2
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0160	Cell Name
LOT No.	06172011	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% fetal bovine serum (GIBCO Cat. # 10099)	Temperature	37 C
Cell Density at Seeding	3.2 - 5.1 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 54 hrs.	Cell Number in Vial (cells/1ml)	6.3 x 10^5
Viability at cell freezing (%)	88.8	Antibiotics Used	free
Passage Number	P16	PDL	+17.5
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), ND.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0160	Cell Name
LOT No.	03042011	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% fetal bovine serum (GIBCO Cat. # 10099)	Temperature	37 C
Cell Density at Seeding	2 - 5 x 10^4 cells/ml	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 2 days	Cell Number in Vial (cells/1ml)	7.2 x 10^5
Viability at cell freezing (%)	90.0	Antibiotics Used	free
Passage Number	P17	PDL	+22.7
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0160	Cell Name
LOT No.	09212010	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% fetal bovine serum (GIBCO Cat. # 10099)	Temperature	37 C
Cell Density at Seeding	2.4 - 6.0 x 10^4 cells/ml	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 3 days.	Cell Number in Vial (cells/1ml)	6.1 x 10^5
Viability at cell freezing (%)	85.9	Antibiotics Used	free
Passage Number	P17	PDL	+24.5
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0160	Cell Name
LOT No.	07262010	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% fetal bovine serum (GIBCO Cat. # 10099)	Temperature	37 C
Cell Density at Seeding	2.2 - 3.5 x 10^4 cells/ml	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 77 hrs	Cell Number in Vial (cells/1ml)	9.9 x 10^5
Viability at cell freezing (%)	87.6	Antibiotics Used	free
Passage Number	P17	PDL	+17.7
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0160	Cell Name
LOT No.	03152010	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% fetal bovine serum (GIBCO Cat. # 10099)	Temperature	37 C
Cell Density at Seeding	2 - 6 x 10^4 cels/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 3-4 days	Cell Number in Vial (cells/1ml)	8.8 x 10^5
Viability at cell freezing (%)	79.0	Antibiotics Used	free
Passage Number	P16	PDL	+17.6
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0160	Cell Name
LOT No.	11122009	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% fetal bovine serum (GIBCO Cat. # 10099, lot 484375)	Temperature	37 C
Cell Density at Seeding	2 - 3 x 10^4 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA
Doubling Time	61 hrs	Cell Number in Vial (cells/1ml)	6.4 x 10^5
Viability at cell freezing (%)	71.9	Antibiotics Used	free
Passage Number	P17	PDL	+17.4
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0160	Cell Name
LOT No.	03022009	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% fetal bovine serum (GIBCO Cat. # 10099. Lot 484375)	Temperature	37 C
Cell Density at Seeding	2 - 3 x 10^4 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA
Doubling Time	53 hrs	Cell Number in Vial (cells/1ml)	4.1 x 10^5
Viability at cell freezing (%)	92.7	Antibiotics Used	free
Passage Number	P18	PDL	+19
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0160	Cell Name
LOT No.	12192008	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% fetal bovine serum (GIBCO Cat.# 10099, Lot 484375)	Temperature	37 C
Cell Density at Seeding	2 - 4 x 10^4 cels/mL	Methods for Passages	0.25% trypsin and 0.02% EDTA. Subculture once a week at split ratio 1/4 is recommended.
Doubling Time	approx. 3 days	Cell Number in Vial (cells/1ml)	5.8 x 10^5
Viability at cell freezing (%)	82.7	Antibiotics Used	free
Passage Number	P17	PDL	+20
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0160	Cell Name
LOT No.	09192008	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% fetal bovine serum (GIBCO Cat.# 10099, lot 484375)	Temperature	37 C
Cell Density at Seeding	2 - 3 x 10^4 cells/mL	Methods for Passages	0.25% trypsin and 0.02% EDTA. Subculture once a week at split ratio 1/4 is recommended.
Doubling Time	approx. 3.5 days	Cell Number in Vial (cells/1ml)	2.8 x 10^5
Viability at cell freezing (%)	75.7	Antibiotics Used	free
Passage Number	P17	PDL	+20
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0160	Cell Name
LOT No.	04022008	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% fetal bovine serum (GIBCO Cat.# 10099, lot 484375)	Temperature	37 C
Cell Density at Seeding	approx. 4 x 10^4 cells/ml (8 x 10^3 cells/cm2)	Methods for Passages	0.25% trypsin and 0.02% EDTA
Doubling Time	approx. 4 days	Cell Number in Vial (cells/1ml)	5.5 x 10^5
Viability at cell freezing (%)	95	Antibiotics Used	free
Passage Number	P17	PDL	+20
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (tyep B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0160	Cell Name
LOT No.	06252007	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS lot; GIBCO 484375)	Temperature	37 C
Cell Density at Seeding	approx. 3 x 10^4 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA. Split ratio = 1:3
Doubling Time	approx. 3.5 days	Cell Number in Vial (cells/1ml)	6.9 x 10^5
Viability at cell freezing (%)	71.2	Antibiotics Used	free
Passage Number	P17	PDL	+20
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD, AST, MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0160	Cell Name
LOT No.	04112007	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS lot; GIBCO 484375)	Temperature	37 C
Cell Density at Seeding	2-4 x 10^4 cells/ml	Methods for Passages	0.25% trypsin and 1 mM EDTA
Doubling Time	approx. 4 days	Cell Number in Vial (cells/1ml)	4 x 10^5
Viability at cell freezing (%)	82	Antibiotics Used	free
Passage Number	P17	PDL	+19
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by G6PD (typeB), NP, LD, MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS-DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0160	Cell Name
LOT No.	11022006	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS lot; GIBCO 4218333S)	Temperature	37 C
Cell Density at Seeding	5 - 6 x 10^4 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA. Split ratio 1/3 is recommended. Change medium every 2 days.
Doubling Time	approx. 3 days	Cell Number in Vial (cells/1ml)	7.6 x 10^5
Viability at cell freezing (%)	95.9	Antibiotics Used	free
Passage Number	P17	PDL	+19 PD
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD, AST.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0160	Cell Name	LI90
LOT No.	11302004	Lot Specification	distribution-1
Medium	DMEM with 10% heat inactivated fetal bovine serum	Temperature	37 C
Cell Density at Seeding	12x10^4 cells/sq.cm	Methods for Passages	Harvested with 0.1% trypsin treatment,subculture 7-10days.Medium renewal every 2 to 3days
Doubling Time	NT	Cell Number in Vial (cells/1ml)	1.3x10^6
Viability at cell freezing (%)	97.2	Antibiotics Used	Free
Passage Number	P15	PDL	+15
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	Culture medium with 5% DMSO	CO2 Conc.	5 %
Viability immediately after thawing (%)		Additional information

Images

Cell No.	JCRB0160	Cell Name	LI90
LOT No.	12092004	Lot Specification	distribution-2
Medium	DMEM	Temperature	37 C
Cell Density at Seeding	1.2x10^4 cells/sq.cm	Methods for Passages	Harvested with 0.1% trypsin　treatment,subculture 7-10days.Medium renewal every 2 to 3days
Doubling Time	NT	Cell Number in Vial (cells/1ml)	1.3x10^6
Viability at cell freezing (%)	98.0	Antibiotics Used	Free
Passage Number	16	PDL	+16
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	Culture medium with 5% DMSO	CO2 Conc.	5 %
Viability immediately after thawing (%)		Additional information

Images

Cell No.	JCRB0160	Cell Name	LI90
LOT No.	02032004	Lot Specification	seed/distribution
Medium	Dulbecco's modifed Eagle's medium with 10% heat inactivated fetal calf serum (Intergen RB52305).	Temperature	37 C
Cell Density at Seeding	1.3 x 10^4 cells/sq.cm.	Methods for Passages	Cells harvested with 0.1% trypsin. Subculture once a week and medium change every 2-3 days.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	1.5 x 10^6
Viability at cell freezing (%)	89.3	Antibiotics Used	free
Passage Number	P17	PDL	+22PD
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	G6PD(invisible), NP, LDH examined. human.
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	Culture medium with 5% DMSO.	CO2 Conc.	5 %
Viability immediately after thawing (%)		Additional information

Images

Cell No.	JCRB0160	Cell Name	LI90
LOT No.	11062003	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% heat inactivated fetal calf serum (Intergen RB52405).	Temperature	37 C
Cell Density at Seeding	1.0 x 10^4 cells/sq.cm.	Methods for Passages	Cells harvested after treatment with 0.1% trypsin. Subculture once a week and medium change every 3-4 days.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	1.9 x 10^6
Viability at cell freezing (%)	94.6	Antibiotics Used	free
Passage Number	P17	PDL	+23PD
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	G6PD(typeB), NP, LDH examined. Human, not HeLa.
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	Culture medium with 5% DMSO.	CO2 Conc.	5 %
Viability immediately after thawing (%)		Additional information

Images

Cell No.	JCRB0160	Cell Name	LI90
LOT No.	10032001	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% heat-inactivated calf serum (Intergen RB52405).	Temperature	37 C
Cell Density at Seeding	1.5 x 10^4 sq.cm.	Methods for Passages	Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	1.4 x 10^6
Viability at cell freezing (%)	95.4	Antibiotics Used	free
Passage Number	P16	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	G6PD(typeB), NP, LDH examined. Human not HeLa.
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	1 to 1 mixture of the culture medium and FM-1 containing 10% DMSO.	CO2 Conc.	5 %
Viability immediately after thawing (%)		Additional information

Images

Cell No.	JCRB0160	Cell Name	LI90
LOT No.	10162001	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% heat inactivated fetal calf serum(Intergen RB52405).	Temperature	37 C
Cell Density at Seeding	1.8 x 10^4 cells/sq.cm.	Methods for Passages	Cells are harvested after treatment with 0.1% trypsin and 0.02% EDTA. Subculture once a week.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	2.1 x 10^6
Viability at cell freezing (%)	96.6	Antibiotics Used	free
Passage Number	P17	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	G6PD(typeB), NP, LDH examined. Human not HeLa.
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	Culture medium containing 10% DMSO.	CO2 Conc.	5 %
Viability immediately after thawing (%)		Additional information

Images

Cell No.	JCRB0160	Cell Name	LI90
LOT No.	09142001	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% heat inactivated fetal calf serum ((ntergen RB52405).	Temperature	37 C
Cell Density at Seeding	5.5-7 x 10^3 cells/sq.cm.	Methods for Passages	Cells are harvested after treatment with 0.1% trypsin and 0.02% EDTA. (Type III trypsin (SIGMA T-89818 was used here).
Doubling Time	NT	Cell Number in Vial (cells/1ml)	1.6 x 10^6
Viability at cell freezing (%)	94.4	Antibiotics Used	free
Passage Number	P12	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	G6PD(typeB), NP, LDH examined. Human not HeLa.
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	Culture medium containing 10% DMSO.	CO2 Conc.	5 %
Viability immediately after thawing (%)		Additional information

Images

Cell No.	JCRB0160	Cell Name	LI90
LOT No.	09032001	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% heat inactivated fetal calf serum.	Temperature	37 C
Cell Density at Seeding	7 x 10^3 cells/sq.cm.	Methods for Passages	Cells harvested after treatment with 0.1% trypsin and 0.02% EDTA.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	2.1 x 10^6
Viability at cell freezing (%)	96.6	Antibiotics Used	free
Passage Number	P12	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	G6PD(typeB), LDH, NP. Human, not a HeLa.
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)	D5S818:11,13 D13S317:11,12 D7S820:10,11 D16S539:9,10 VWA:16,17 TH01:9 AM:X TPOX:8,11 CSF1PO:9,12
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	Culture medium with 5% DMSO.	CO2 Conc.	5 %
Viability immediately after thawing (%)		Additional information

Images

Cell No.	JCRB0160	Cell Name	LI90
LOT No.	08162001	Lot Specification	seed(distribution)
Medium	Dulbecco's modified Eagle's medium with 10% heat inactivated fetal calf serum.	Temperature	37 C
Cell Density at Seeding	3.8 x 10^3 cells/sq.cm.	Methods for Passages	Cells are harvested after treatment with 0.1% type III trypsin and 0.02% EDTA. Medium change twice a week. Subculture once a week.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	1.4 x 10^6
Viability at cell freezing (%)	95.4	Antibiotics Used	free
Passage Number	P9	PDL	10PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	G6PD(typeB), LDH, NP. Human, not a HeLa.
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	1 to 1 mixture of culture medium and FM-1 medium containing 10% DMSO.	CO2 Conc.	5 %
Viability immediately after thawing (%)		Additional information

Images

Cell No.	JCRB0160	Cell Name	LI90
LOT No.	08172001	Lot Specification	seed(distribution)
Medium	Dulbecco's modified Eagle's medium with 10% heat inactivated fetal calf serum.	Temperature	37 C
Cell Density at Seeding	3.8 x 10^3 cells/sq.cm.	Methods for Passages	Cells are harvested after treatment with 0.1% type III trypsin and 0.02% EDTA. Medium change twice a week. Subculture once a week.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	1.2 x 10^6
Viability at cell freezing (%)	96.7	Antibiotics Used	free
Passage Number	P9	PDL	10PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	G6PD(typeB), LDH, NP. Human, not a HeLa.
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	1 to 1 mixture of culture medium and FM-1 medium containing 10% DMSO.	CO2 Conc.	5 %
Viability immediately after thawing (%)		Additional information

Images

Cell No.	JCRB0160	Cell Name	LI90
LOT No.	06062001	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% heat inactivated fetal calf serum (Intergen RB52405).	Temperature	37 C
Cell Density at Seeding	5.0 x 10^3 cells/sq.cm.	Methods for Passages	Cells harvested after treatment with 0.25% trypsin and 1mM EDTA (-PBS) at 37 C for 10 min. (Alternative: 2.5% trypsin at room temp. for 3 min.)
Doubling Time	NT	Cell Number in Vial (cells/1ml)	9.8 x 10^5
Viability at cell freezing (%)	93.4	Antibiotics Used	free
Passage Number	P10*	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	G6PD(typeB), NP, LDH examined. Human.
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	Culture medium containing 5% DMSO.	CO2 Conc.	5 %
Viability immediately after thawing (%)		Additional information

Images

Cell No.	JCRB0160	Cell Name	LI90
LOT No.	04172001	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% heat inactivated fetal calf serum.	Temperature	37 C
Cell Density at Seeding	4.5 x 10^3 cells/sq.cm.	Methods for Passages	Cells are harvested after treatment with 0.25% trypsin and 1mM EDTA. Medium change twice a week and Subculture once a week.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	1.7 x 10^6
Viability at cell freezing (%)	56.0	Antibiotics Used	free
Passage Number	P8*	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	G6PD(typeB), NP, LDH. Human, not HeLa.
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	Culture medium containing 5% DMSO.	CO2 Conc.	5 %
Viability immediately after thawing (%)		Additional information

Images

Cell No.	JCRB0160	Cell Name	LI90
LOT No.	08212013	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% heat-inactivated fetal bovine serum (FBS; GIBCO Cat. # 10099).	Temperature	37C
Cell Density at Seeding	approx. 2.6 x 10^4 cells/mL	Methods for Passages	Cells harvested after treatment with PBS containing 0.25% trypsin and 0.02% EDTA. Change medium twice a week.
Doubling Time		Cell Number in Vial (cells/1ml)	8.3 x 10^5 (cells/1m
Viability at cell freezing (%)		Antibiotics Used	free
Passage Number	P16	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	G6PD(typeB), NP, MD were examined. Human,not HeLa
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing	Culture medium with 10% DMSO	CO2 Conc.	5%
Viability immediately after thawing (%)	93.3%	Additional information

Cell No.	JCRB0160	Cell Name	LI90
LOT No.	09082017	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% heat inactivated fetal bovine serum(SIGMA12J396)	Temperature	37 C
Cell Density at Seeding	7.00-1.30 cells/sq.cm	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 1mM EDTA.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	1.56x10^6
Viability at cell freezing (%)	98.73	Antibiotics Used	free
Passage Number	p16	PDL	20.22
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)	D5S818:11,13 D13S317:12 D7S820:10,11 D16S539:9,10 VWA:16,17 TH01:9 AM:X TPOX:8,11 CSF1PO:9,12
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD)	CO2 Conc.	5%
Viability immediately after thawing (%)	NT	Additional information

Cell No.	JCRB0160	Cell Name	LI90
LOT No.	02282020	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012)	Temperature	37 C
Cell Density at Seeding	1.5 - 6.0 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 58 hrs.	Cell Number in Vial (cells/1ml)	7.6 x 10^5
Viability at cell freezing (%)	98	Antibiotics Used	free
Passage Number	P16	PDL	+18.8
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	89	Additional information

Cell No.	JCRB0160	Cell Name	LI90
LOT No.	05222023	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium (low glucose) with 10% fetal bovine serum (FBS; GIBCO Cat. # 10437)	Temperature	37 C
Cell Density at Seeding	2.4 - 7.9 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 3 days.	Cell Number in Vial (cells/1ml)	4.2 x 10^5
Viability at cell freezing (%)	99	Antibiotics Used	free
Passage Number	P17	PDL	+16.2
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:11,13 D13S317:12 D7S820:10,11 D16S539:9,10 VWA:16,17 TH01:9 AM:X TPOX:8,11 CSF1PO:9,12
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	82	Additional information

JCRB Cell Bank

JCRB0160 LI90

Cell information

LOT Information