JCRB0160 LI90

Cell information

Important Notice(s)

Notice for the cultivation of the LI90 cells (JCRB0160) / LI90の培養上の注意

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0160 Cell Name   LI90
Profile   Ito (fat-storing) cells, stellate-shaped mesenchymal cells that exist in the space of Disse of the liver and contain many fat droplets in cytoplasm. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   55
Identity   available Tissue for Primary Cancer   liver, gallbladder
Case history   epithelioid hemangioendothelioma. Metastasis   
Tissue Metastasized    Genetics   Karyology: 47XX, 6q-, +22
Life Span   finite Crisis PDL   
Morphology   epithelial-like Character   
Classify   tumor Established by   Murakami,K. and etc.
Registered by   Matsuura,T. Regulation for Distribution   free
Comment    Year   2000
Medium   Dulbecco's modified Eagle's medium with 10% heat-inactivated fetal bovine serum. Methods for Passages   Cells harvested after treatment with PBS containing 0.25% trypsin and 1 mM EDTA. Change medium twice a week.
Cell Number on Passage   Not specified. Race   Japanese
CO2 Conc.   5 % Tissue Sampling   liver
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0390
Reference
Pubmed id:12811248 Establishment of immortalized human hepatic stellate scavenger cells to develop bioartificial livers.
Watanabe T,Shibata N,Westerman KA,Okitsu T,Allain JE,Sakaguchi M,Totsugawa T,Maruyama M,Matsumura T,Noguchi H,Yamamoto S,Hikida M,Ohmori A,Reth M,Weber A,Tanaka N,Leboulch P,Kobayashi N
Transplantation. 2003 Jun 15;75(11):1873-80
Pubmed id:10462172 Retinol esterification activity contributes to retinol transport in stellate cells.
Matsuura T,Hasumura S,Nagamori S,Murakami K
Cell Struct Funct. 1999 Jun;24(3):111-6
Pubmed id:7540235 Establishment of a new human cell line, LI90, exhibiting characteristics of hepatic Ito (fat-storing) cells.
Murakami K,Abe T,Miyazawa M,Yamaguchi M,Masuda T,Matsuura T,Nagamori S,Takeuchi K,Abe K,Kyogoku M
Lab Invest. 1995 Jun;72(6):731-9

Images
Movies



LOT Information

Cell No.   JCRB0160 Cell Name   
LOT No.   06082012 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   2.6 - 5.5 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 2 days Cell Number in Vial (cells/1ml)   2.8 x 10^5
Viability at cell freezing (%)   93.9 Antibiotics Used   free
Passage Number   P16 PDL   +18.4
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0160 Cell Name   
LOT No.   11072011 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (1 g/L glucose) with 10% fetal bovine serum (FBS, GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   2 - 5 x 10^4 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 3-4 days Cell Number in Vial (cells/1ml)   2.6 x 10^5
Viability at cell freezing (%)   68.4 Antibiotics Used   free
Passage Number   P17 PDL   +21.2
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0160 Cell Name   
LOT No.   06172011 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   3.2 - 5.1 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 54 hrs. Cell Number in Vial (cells/1ml)   6.3 x 10^5
Viability at cell freezing (%)   88.8 Antibiotics Used   free
Passage Number   P16 PDL   +17.5
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), ND.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0160 Cell Name   
LOT No.   03042011 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   2 - 5 x 10^4 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 2 days Cell Number in Vial (cells/1ml)   7.2 x 10^5
Viability at cell freezing (%)   90.0 Antibiotics Used   free
Passage Number   P17 PDL   +22.7
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0160 Cell Name   
LOT No.   09212010 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   2.4 - 6.0 x 10^4 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 3 days. Cell Number in Vial (cells/1ml)   6.1 x 10^5
Viability at cell freezing (%)   85.9 Antibiotics Used   free
Passage Number   P17 PDL   +24.5
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0160 Cell Name   
LOT No.   07262010 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   2.2 - 3.5 x 10^4 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 77 hrs Cell Number in Vial (cells/1ml)   9.9 x 10^5
Viability at cell freezing (%)   87.6 Antibiotics Used   free
Passage Number   P17 PDL   +17.7
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0160 Cell Name   
LOT No.   03152010 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   2 - 6 x 10^4 cels/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 3-4 days Cell Number in Vial (cells/1ml)   8.8 x 10^5
Viability at cell freezing (%)   79.0 Antibiotics Used   free
Passage Number   P16 PDL   +17.6
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0160 Cell Name   
LOT No.   11122009 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (GIBCO Cat. # 10099, lot 484375) Temperature   37 C
Cell Density at Seeding   2 - 3 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   61 hrs Cell Number in Vial (cells/1ml)   6.4 x 10^5
Viability at cell freezing (%)   71.9 Antibiotics Used   free
Passage Number   P17 PDL   +17.4
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0160 Cell Name   
LOT No.   03022009 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (GIBCO Cat. # 10099. Lot 484375) Temperature   37 C
Cell Density at Seeding   2 - 3 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   53 hrs Cell Number in Vial (cells/1ml)   4.1 x 10^5
Viability at cell freezing (%)   92.7 Antibiotics Used   free
Passage Number   P18 PDL   +19
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0160 Cell Name   
LOT No.   12192008 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (GIBCO Cat.# 10099, Lot 484375) Temperature   37 C
Cell Density at Seeding   2 - 4 x 10^4 cels/mL Methods for Passages   0.25% trypsin and 0.02% EDTA. Subculture once a week at split ratio 1/4 is recommended.
Doubling Time   approx. 3 days Cell Number in Vial (cells/1ml)   5.8 x 10^5
Viability at cell freezing (%)   82.7 Antibiotics Used   free
Passage Number   P17 PDL   +20
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0160 Cell Name   
LOT No.   09192008 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (GIBCO Cat.# 10099, lot 484375) Temperature   37 C
Cell Density at Seeding   2 - 3 x 10^4 cells/mL Methods for Passages   0.25% trypsin and 0.02% EDTA. Subculture once a week at split ratio 1/4 is recommended.
Doubling Time   approx. 3.5 days Cell Number in Vial (cells/1ml)   2.8 x 10^5
Viability at cell freezing (%)   75.7 Antibiotics Used   free
Passage Number   P17 PDL   +20
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0160 Cell Name   
LOT No.   04022008 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (GIBCO Cat.# 10099, lot 484375) Temperature   37 C
Cell Density at Seeding   approx. 4 x 10^4 cells/ml (8 x 10^3 cells/cm2) Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 4 days Cell Number in Vial (cells/1ml)   5.5 x 10^5
Viability at cell freezing (%)   95 Antibiotics Used   free
Passage Number   P17 PDL   +20
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (tyep B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0160 Cell Name   
LOT No.   06252007 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS lot; GIBCO 484375) Temperature   37 C
Cell Density at Seeding   approx. 3 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. Split ratio = 1:3
Doubling Time   approx. 3.5 days Cell Number in Vial (cells/1ml)   6.9 x 10^5
Viability at cell freezing (%)   71.2 Antibiotics Used   free
Passage Number   P17 PDL   +20
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD, AST, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0160 Cell Name   
LOT No.   04112007 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS lot; GIBCO 484375) Temperature   37 C
Cell Density at Seeding   2-4 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 1 mM EDTA
Doubling Time   approx. 4 days Cell Number in Vial (cells/1ml)   4 x 10^5
Viability at cell freezing (%)   82 Antibiotics Used   free
Passage Number   P17 PDL   +19
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by G6PD (typeB), NP, LD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS-DMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0160 Cell Name   
LOT No.   11022006 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   5 - 6 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. Split ratio 1/3 is recommended. Change medium every 2 days.
Doubling Time   approx. 3 days Cell Number in Vial (cells/1ml)   7.6 x 10^5
Viability at cell freezing (%)   95.9 Antibiotics Used   free
Passage Number   P17 PDL   +19 PD
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0160 Cell Name   LI90
LOT No.   11302004 Lot Specification   distribution-1
Medium   DMEM with 10% heat inactivated fetal bovine serum Temperature   37 C
Cell Density at Seeding   12x10^4 cells/sq.cm Methods for Passages   Harvested with 0.1% trypsin treatment,subculture 7-10days.Medium renewal every 2 to 3days
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.3x10^6
Viability at cell freezing (%)   97.2 Antibiotics Used   Free
Passage Number   P15 PDL   +15
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0160 Cell Name   LI90
LOT No.   12092004 Lot Specification   distribution-2
Medium   DMEM Temperature   37 C
Cell Density at Seeding   1.2x10^4 cells/sq.cm Methods for Passages   Harvested with 0.1% trypsin treatment,subculture 7-10days.Medium renewal every 2 to 3days
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.3x10^6
Viability at cell freezing (%)   98.0 Antibiotics Used   Free
Passage Number   16 PDL   +16
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0160 Cell Name   LI90
LOT No.   02032004 Lot Specification   seed/distribution
Medium   Dulbecco's modifed Eagle's medium with 10% heat inactivated fetal calf serum (Intergen RB52305). Temperature   37 C
Cell Density at Seeding   1.3 x 10^4 cells/sq.cm. Methods for Passages   Cells harvested with 0.1% trypsin. Subculture once a week and medium change every 2-3 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.5 x 10^6
Viability at cell freezing (%)   89.3 Antibiotics Used   free
Passage Number   P17 PDL   +22PD
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(invisible), NP, LDH examined. human.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0160 Cell Name   LI90
LOT No.   11062003 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% heat inactivated fetal calf serum (Intergen RB52405). Temperature   37 C
Cell Density at Seeding   1.0 x 10^4 cells/sq.cm. Methods for Passages   Cells harvested after treatment with 0.1% trypsin. Subculture once a week and medium change every 3-4 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.9 x 10^6
Viability at cell freezing (%)   94.6 Antibiotics Used   free
Passage Number   P17 PDL   +23PD
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB), NP, LDH examined. Human, not HeLa.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0160 Cell Name   LI90
LOT No.   10032001 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% heat-inactivated calf serum (Intergen RB52405). Temperature   37 C
Cell Density at Seeding   1.5 x 10^4 sq.cm. Methods for Passages   Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   95.4 Antibiotics Used   free
Passage Number   P16 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB), NP, LDH examined. Human not HeLa.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   1 to 1 mixture of the culture medium and FM-1 containing 10% DMSO. CO2 Conc.   5 %
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0160 Cell Name   LI90
LOT No.   10162001 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% heat inactivated fetal calf serum(Intergen RB52405). Temperature   37 C
Cell Density at Seeding   1.8 x 10^4 cells/sq.cm. Methods for Passages   Cells are harvested after treatment with 0.1% trypsin and 0.02% EDTA. Subculture once a week.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.1 x 10^6
Viability at cell freezing (%)   96.6 Antibiotics Used   free
Passage Number   P17 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB), NP, LDH examined. Human not HeLa.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium containing 10% DMSO. CO2 Conc.   5 %
RFLP   Unique Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0160 Cell Name   LI90
LOT No.   09142001 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% heat inactivated fetal calf serum ((ntergen RB52405). Temperature   37 C
Cell Density at Seeding   5.5-7 x 10^3 cells/sq.cm. Methods for Passages   Cells are harvested after treatment with 0.1% trypsin and 0.02% EDTA. (Type III trypsin (SIGMA T-89818 was used here).
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.6 x 10^6
Viability at cell freezing (%)   94.4 Antibiotics Used   free
Passage Number   P12 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB), NP, LDH examined. Human not HeLa.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium containing 10% DMSO. CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0160 Cell Name   LI90
LOT No.   09032001 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% heat inactivated fetal calf serum. Temperature   37 C
Cell Density at Seeding   7 x 10^3 cells/sq.cm. Methods for Passages   Cells harvested after treatment with 0.1% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.1 x 10^6
Viability at cell freezing (%)   96.6 Antibiotics Used   free
Passage Number   P12 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB), LDH, NP. Human, not a HeLa.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,13
D13S317:11,12
D7S820:10,11
D16S539:9,10
VWA:16,17
TH01:9
AM:X
TPOX:8,11
CSF1PO:9,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0160 Cell Name   LI90
LOT No.   08162001 Lot Specification   seed(distribution)
Medium   Dulbecco's modified Eagle's medium with 10% heat inactivated fetal calf serum. Temperature   37 C
Cell Density at Seeding   3.8 x 10^3 cells/sq.cm. Methods for Passages   Cells are harvested after treatment with 0.1% type III trypsin and 0.02% EDTA. Medium change twice a week. Subculture once a week.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   95.4 Antibiotics Used   free
Passage Number   P9 PDL   10PDL
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB), LDH, NP. Human, not a HeLa.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   1 to 1 mixture of culture medium and FM-1 medium containing 10% DMSO. CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0160 Cell Name   LI90
LOT No.   08172001 Lot Specification   seed(distribution)
Medium   Dulbecco's modified Eagle's medium with 10% heat inactivated fetal calf serum. Temperature   37 C
Cell Density at Seeding   3.8 x 10^3 cells/sq.cm. Methods for Passages   Cells are harvested after treatment with 0.1% type III trypsin and 0.02% EDTA. Medium change twice a week. Subculture once a week.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)   96.7 Antibiotics Used   free
Passage Number   P9 PDL   10PDL
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB), LDH, NP. Human, not a HeLa.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   1 to 1 mixture of culture medium and FM-1 medium containing 10% DMSO. CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0160 Cell Name   LI90
LOT No.   06062001 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% heat inactivated fetal calf serum (Intergen RB52405). Temperature   37 C
Cell Density at Seeding   5.0 x 10^3 cells/sq.cm. Methods for Passages   Cells harvested after treatment with 0.25% trypsin and 1mM EDTA (-PBS) at 37 C for 10 min. (Alternative: 2.5% trypsin at room temp. for 3 min.)
Doubling Time   NT Cell Number in Vial (cells/1ml)   9.8 x 10^5
Viability at cell freezing (%)   93.4 Antibiotics Used   free
Passage Number   P10* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB), NP, LDH examined. Human.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium containing 5% DMSO. CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0160 Cell Name   LI90
LOT No.   04172001 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% heat inactivated fetal calf serum. Temperature   37 C
Cell Density at Seeding   4.5 x 10^3 cells/sq.cm. Methods for Passages   Cells are harvested after treatment with 0.25% trypsin and 1mM EDTA. Medium change twice a week and Subculture once a week.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)   56.0 Antibiotics Used   free
Passage Number   P8* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB), NP, LDH. Human, not HeLa.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium containing 5% DMSO. CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0160 Cell Name   LI90
LOT No.   08212013 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% heat-inactivated fetal bovine serum (FBS; GIBCO Cat. # 10099). Temperature   37C
Cell Density at Seeding   approx. 2.6 x 10^4 cells/mL Methods for Passages   Cells harvested after treatment with PBS containing 0.25% trypsin and 0.02% EDTA. Change medium twice a week.
Doubling Time    Cell Number in Vial (cells/1ml)   8.3 x 10^5 (cells/1m
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   P16 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB), NP, MD were examined. Human,not HeLa
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   Culture medium with 10% DMSO CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   93.3%
Additional information   
Cell No.   JCRB0160 Cell Name   LI90
LOT No.   09082017 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   7.00-1.30 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 1mM EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.56x10^6
Viability at cell freezing (%)   98.73 Antibiotics Used   free
Passage Number   p16 PDL   20.22
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,13
D13S317:12
D7S820:10,11
D16S539:9,10
VWA:16,17
TH01:9
AM:X
TPOX:8,11
CSF1PO:9,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   NT
Additional information   
Cell No.   JCRB0160 Cell Name   LI90
LOT No.   02282020 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1.5 - 6.0 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 58 hrs. Cell Number in Vial (cells/1ml)   7.6 x 10^5
Viability at cell freezing (%)   98 Antibiotics Used   free
Passage Number   P16 PDL   +18.8
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   89
Additional information   
CLOSE