JCRB0151.1 p53-def-MOSE

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0151.1 Cell Name   p53-def-MOSE
Profile   ovarian surface epithelial cell line from p53(-/-) mouse Other Name   
Animal   mouse Strain   
Genus   Mus Species   musclus
Sex   F Age   
Identity    Tissue for Primary Cancer   ovary
Case history   p53 gene deleted mouse Metastasis   
Tissue Metastasized    Genetics   p53 gene was homozygously deleted, laminin positive.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like, cobble stone like. Character   non-tumorigenic in nude mice
Classify   normal Established by   Kido,M. & Shibuya,M.
Registered by   Kido,M. Regulation for Distribution   
Comment   Adhesion efficiency 100%. Anchorage dependency is yes. Growth temperature is 37 C and CO2 concentration is 5%. Freezing medium contains 10% DMSO. Year   1999
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum. Methods for Passages   0.25% trypsin treatment require to harvest cells.
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   ovarian surface epitherium
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_C563
Reference
Pubmed id:9820870Isolation and characterization of mouse ovarian surface epithelial cell lines.
Kido M,Shibuya M
Pathol Res Pract. 1998;194(10):725-30
Pubmed id:7504233Enhanced proliferative potential in culture of cells from p53-deficient mice.
Tsukada T,Tomooka Y,Takai S,Ueda Y,Nishikawa S,Yagi T,Tokunaga T,Takeda N,Suda Y,Abe S
Oncogene. 1993 Dec;8(12):3313-22

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LOT Information

Cell No.   JCRB0151.1 Cell Name   
LOT No.   09242002 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS lot; BioWhittaker 0S069F) Temperature   37 C
Cell Density at Seeding   1-1.5 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS
Doubling Time   ca. 24 hrs Cell Number in Vial (cells/1ml)   7.8 x 10^5
Viability at cell freezing (%)   87.8 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mous by NP, G6PD, MD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS-DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
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