IFO50003 CKT-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50003 Cell Name   CKT-1
Profile   This cell line is useful to produce and assay bovine adinovirus 3 in vitro. (J. Virol., 16, 621 (1975)). CKT-1 is a clone of CKT. Other Name   
Animal   bovine Strain   
Genus   Bos Species   taurus
Sex    Age   
Identity    Tissue for Primary Cancer   kidney
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   useful to assay bovine adenovirus 3
Classify   normal Established by   Kimura, S.
Registered by   Igarashi,K. Regulation for Distribution   
Comment   This line is useful as indicator cells to assay mycoplasmal contamination in cell cultures. Year   1985
Medium   Eagle's minimal essential medium with 10% fetal bovine serum Methods for Passages   0.25% trypsin and 0.02% EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   kidney
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2403
Reference
Pubmed id:4303468A new cell line of calf kidney and its susceptibility to viruses.
Kimura S,Fukui K,Yoshida N,Matsubara Y
Jpn J Microbiol. 1968 Sep;12(3):293-8

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LOT Information

Cell No.   IFO50003 Cell Name   CKT-1
LOT No.   427 Lot Specification   distribution
Medium   E.MEM, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   3 x 10^6
Viability at cell freezing (%)   93 Antibiotics Used   free
Passage Number   Unknown (12 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD, MD, MPI
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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