-Detailed Information [IFO50005]-

IFO50005 J-111

Cell information

Important Notice(s)

JCRB0073, IFO50005 J-111 is HeLa-derivative / H-111細胞のHeLa細胞汚染

[Problem of J-111 cell line: This cell line is HeLa-derivative.]

J-111 cell line was established as a human acute monocytic leukemia cell line. However, later studies by chromosome analysis and by DNA profiling revealed the J-111 cell line is a subline of HeLa (human uterine cervical carcinoma-derived cell line).
This replacement is worldwide, and therefore the replacement (or cross contamination) occurred early in the establishment of J-111.
This cell line has been used for many studies, and therefore cell bank does not stop the distribution of this cell line.
But it should not be used for the studies of liver cells.

ご注意

J-111細胞(JCRB0073, IFO50005)は、ヒト血液由来細胞として樹立されましたが、現在世界的に広くHeLa細胞に汚染されてしまっている事がわかっております。

一方本細胞は、広く用いられている故にHeLa細胞の一亜株として研究に利用される場合があります。このため、細胞バンクでは本細胞の登録を抹消しておりません。

本細胞がHeLaに汚染されている事実はカタログやインターネット上でHeLa markerとして記載しておりますが、念のためご注意を促しております。血球細胞としてのご使用はさけるよう、お願い申し上げます。

[References]
参考文献

Hay,R.J. et al. (ed.) Catalogue of Cell Lines and Hybridomas. Rockville, ATCC, (1988)

Homma,M. et al. A new DNA profiling system for cell line identification for use in cell banks in Japan. In Vitro Cell. Dev. Biol., 28, 24-28, (1992)

Lavappa,K.S. et al. Cyto genetic investigations of long-term human hetero ploid cell lines J-111 detroit-98-CCL18.2 and detroit-98-CCL18.3 with HeLa characteristics. Am. J. Hum. Genet., 29: 67A, (1977)

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Cell type：general cells (View Pricing Information)

JCRB No.	IFO50005	Cell Name	J-111
Profile	HeLa markers*	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex	F	Age
Identity	available	Tissue for Primary Cancer	HeLa-contaminant
Case history		Metastasis
Tissue Metastasized		Genetics	HeLa markers observed.
Life Span	infinite	Crisis PDL
Morphology	epithelial-like	Character	originally established from acute monocytic leukemia, HeLa-contaminated
Classify	tumor	Established by	Osgood, E. E. & Brooke, J. H.
Registered by	Takeuchi,M.	Regulation for Distribution
Comment	*Contamination of HeLa cells has been suspected in this line. This cell line is different from the CRL-8129 in ATCC although the name is same.	Year	1985
Medium	Eagle's minimal essential medium with 10% fetal bovine serum	Methods for Passages	trypsin-EDTA
Cell Number on Passage		Race
CO2 Conc.	5 %	Tissue Sampling	blood
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	not tested				Detected RNA Virus	not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_2965

Reference
Pubmed id:14185313	ANIMAL CELL STRAINS. THE CELL CULTURE COLLECTION COMMITTEE HAS ASSEMBLED AND CERTIFIED 23 STRAINS OF ANIMAL CELLS. LEDLEY RS Science. 1964 Oct 9;146(3641):241-3
Pubmed id:13260360	Continuous tissue culture of leukocytes from human leukemic bloods by application of gradient principles. OSGOOD EE,BROOKE JH Blood. 1955 Oct;10(10):1010-22

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LOT Information

Cell No.	IFO50005	Cell Name	J-111
LOT No.	74	Lot Specification	distribution
Medium	E.MEM, 90% ; FBS, 10%	Temperature	37 C
Cell Density at Seeding		Methods for Passages	trypsin-EDTA
Doubling Time		Cell Number in Vial (cells/1ml)	4 x 10^6
Viability at cell freezing (%)		Antibiotics Used	free
Passage Number	Unknown (>6 at IFO)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NP, G6PD, MD, MPI
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing	10% DMSO, 20% serum (final conc.) - culture medium	CO2 Conc.
Viability immediately after thawing (%)		Additional information

JCRB Cell Bank

IFO50005 J-111

Cell information

LOT Information