IFO50010 C6/36

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50010 Cell Name   C6/36
Profile   This cell line is useful for replication of flavoviruses and dengue virus. Other Name   
Animal   mosquito Strain   
Genus   Aedes Species   albopictus
Sex    Age   
Identity    Tissue for Primary Cancer   hatched larvae
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   useful for replication of flavivirus
Classify    Established by   Singh, K. R. P.
Registered by   Ito,H. Regulation for Distribution   
Comment    Year   1985
Medium   Eagle's minimal essential medium with 10% fetal bovine serum. Cultivation temperature = 28 C. Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   hatched larvae
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_Z230
Reference
Pubmed id:690610Isolation of a Singh's Aedes albopictus cell clone sensitive to Dengue and Chikungunya viruses.
Igarashi A
J Gen Virol. 1978 Sep;40(3):531-44
Pubmed id:noneCell cultures derived from larvae of Aedes albopictus (Skuse) and Aedes aegypti (L.).
Singh KRP.
Curr. Sci. 36: 506-508, 1967.
Pubmed id:noneEffect of incubation temperature on dengue-2 viral antigen production and viral RNA synthesis in infected Aedes albopictus clone C6/36 cell line
Marlou Noel M. MANGADA, Halimah MOHAMED, Leticia del Carmen CASTILLO, Futoshi HASEBE, Akira IGARASHI
Tiss Cult Res Commun. 14: 213-220, 1995

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LOT Information

Cell No.   IFO50010 Cell Name   
LOT No.   01282008 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum. Cultivate at 28 C. Temperature   28 C
Cell Density at Seeding   10^5 cells/ml Methods for Passages   0.25% trypsin adn 0.02% EDTA
Doubling Time   30 - 40 hrs Cell Number in Vial (cells/1ml)   3.2 x 10^6
Viability at cell freezing (%)   80 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Tested with G6PD, NP and MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50010 Cell Name   C6/36
LOT No.   853 Lot Specification   distribution
Medium   E.MEM, 90% ; FBS, 10% Temperature   28 C
Cell Density at Seeding    Methods for Passages   tyrpsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   1 x 10^6
Viability at cell freezing (%)   98 Antibiotics Used   free
Passage Number   Unknown (8 at IFO) PDL   
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50010 Cell Name   C6/36
LOT No.   12052017 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Cultivation temperature = 28 C. Temperature   28 C
Cell Density at Seeding   1.2 - 1.7 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 36 hrs. Cell Number in Vial (cells/1ml)   5.8 x 10^6
Viability at cell freezing (%)   89 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   89 Additional information   
Cell No.   IFO50010 Cell Name   C6/36
LOT No.   07222024 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K). Cultivation temperature = 28 C. Temperature   28 C
Cell Density at Seeding   0.5 - 1.3 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 35 hrs. Cell Number in Vial (cells/1ml)   6.4 x 10^6
Viability at cell freezing (%)   96 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   95 Additional information   
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