IFO50013 RPMI 8226

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50013 Cell Name   RPMI 8226
Profile   Ig lambda light chain-secreting. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   61
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   multiple myeloma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphoblast-like Character   myeloma, multiple
Classify   tumor Established by   Moore, G.
Registered by   Takeuchi,M. Regulation for Distribution   
Comment    Year   1985
Medium   RPMI1640 medium with 10% fetal bovine serum Methods for Passages   dilution
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   peripheral blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0014
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 905964
Reference
Pubmed id:5243066Cell line derived from patient with myeloma.
Moore GE,Kitamura H
N Y State J Med. 1968 Aug 1;68(15):2054-60

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LOT Information

Cell No.   IFO50013 Cell Name   RPMI 8226
LOT No.   87 Lot Specification   distribution
Medium   RPMI 1640, 90% ; FBS, 10% // "F87R25, 50013" on vial label Temperature   37 C
Cell Density at Seeding    Methods for Passages   dilution
Doubling Time    Cell Number in Vial (cells/1ml)   5 x 10^6
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   Unknown (7 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD, MD, MPI, PepB
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   [F87R25, 50013] on vial label
Cell No.   IFO50013 Cell Name   RPMI 8226
LOT No.   11082017 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   approx. 2 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture)
Doubling Time   approx. 48 hrs. Cell Number in Vial (cells/1ml)   2.9 x 10^6
Viability at cell freezing (%)   75 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   75 Additional information   
Cell No.   IFO50013 Cell Name   RPMI 8226
LOT No.   05212021 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   2.9 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture)
Doubling Time   approx. 2 days Cell Number in Vial (cells/1ml)   3.8 x 10^6
Viability at cell freezing (%)   93 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   89 Additional information   
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