IFO50015 F2408

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50015 Cell Name   F2408
Profile   Rat fibroblast cell line. Other Name   
Animal   rat Strain   Fischer
Genus   Rattus Species   norvegicus
Sex    Age   embryo
Identity    Tissue for Primary Cancer   embryo
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   fibroblast-like
Classify    Established by   Eckhalt, W.
Registered by   Hakura,A. Regulation for Distribution   
Comment    Year   1985
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   embryo, whole
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_8352
Reference
Pubmed id:6307528Cellular responsiveness to growth factors correlates with a cell's ability to express the transformed phenotype.
Kaplan PL,Ozanne B
Cell. 1983 Jul;33(3):931-8

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LOT Information

Cell No.   IFO50015 Cell Name   F2408
LOT No.   1138 Lot Specification   distribution
Medium   D.MEM, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding   0.5-1.0 x 10^4 cells/ml Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   2.5 x 10^6
Viability at cell freezing (%)   90 Antibiotics Used   free
Passage Number   Unknown (4 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD, MD, LD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.)-culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50015 Cell Name   
LOT No.   1137 Lot Specification   distribution
Medium   D.MEM, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding   0.5-1.0 x 10^4 cells/ml Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   2.5 x 10^6
Viability at cell freezing (%)   90 Antibiotics Used   free
Passage Number   Unknown (4 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD, MD, LD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.)-culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50015 Cell Name   F2408
LOT No.   11272013 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   0.7 - 1.5 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   3.5 x 10^6
Viability at cell freezing (%)   95.1 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as rat by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   95.1 Additional information   
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