IFO50016 Chang Liver
Cell information
Important Notice(s)IFO50016 Chang Liver is HeLa-derivative.
Cell type：general cells (View Pricing Information)
| JCRB No. | IFO50016 | Cell Name | Chang Liver |
|---|---|---|---|
| Profile | Hela markers* | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | Age | ||
| Identity | available | Tissue for Primary Cancer | HeLa-contaminant |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | originally established from liver, HeLa-contaminated |
| Classify | tumor | Established by | Chang, R. S. |
| Registered by | Takeuchi,M. | Regulation for Distribution | |
| Comment | *Contamination of HeLa cells has been suspected in this line. | Year | 1985 |
| Medium | Eagle's minimal essential medium with 10% fetal bovine serum | Methods for Passages | trypsin-EDTA |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5 % | Tissue Sampling | liver |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:1701344 | Production of interleukin 6 from human liver cell lines: production of interleukin 6 is not concurrent with the production of alpha-fetoprotein. Matsuguchi T,Okamura S,Kawasaki C,Niho Y Cancer Res. 1990 Dec 1;50(23):7457-9 |
| Pubmed id:856839 | Expression of liver and placental alkaline phosphatases in Chang liver cells. Ludueña MA,Iverson GM,Sussman HH J Cell Physiol. 1977 Apr;91(1):119-29 |
| Pubmed id:14185313 | ANIMAL CELL STRAINS. THE CELL CULTURE COLLECTION COMMITTEE HAS ASSEMBLED AND CERTIFIED 23 STRAINS OF ANIMAL CELLS. LEDLEY RS Science. 1964 Oct 9;146(3641):241-3 |
| Pubmed id:14477443 | Clonal variation and interaction of cells with viruses. MURPHY WH,LANDAU BJ Natl Cancer Inst Monogr. 1962 Apr;7():249-71 |
| Pubmed id:13237268 | Continuous subcultivation of epithelial-like cells from normal human tissues. CHANG RS Proc Soc Exp Biol Med. 1954 Nov;87(2):440-3 |
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LOT Information
| Cell No. | IFO50016 | Cell Name | Chang Liver |
|---|---|---|---|
| LOT No. | 328 | Lot Specification | distribution |
| Medium | E.MEM, 90% ; FBS, 10% | Temperature | 37 C |
| Cell Density at Seeding | Methods for Passages | trypsin-EDTA | |
| Doubling Time | Cell Number in Vial (cells/1ml) | 1 x 10^6 | |
| Viability at cell freezing (%) | >90 | Antibiotics Used | free |
| Passage Number | Unknown (6 at IFO) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NP, G6PD, MD, LD |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | D5S818:11,12 D13S317:12,14 D7S820:8,12 D16S539:9,10 VWA:16,18 TH01:7 AM:X TPOX:8,12 CSF1PO:9,10 |
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| Adhesion | Exoteric Gene | ||
| Medium for Freezing | 10% DMSO, 20% serum (final conc.) - culture medium | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information |
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