IFO50036 P388D1 Cell information
Cell type:general cells
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JCRB No.
IFO50036
Cell Name
P388D1
Profile
lymphoid neoplasm, derived from P388, (DBA/2 mouse)
Other Name
Animal
mouse
Strain
DBA/2
Genus
Mus
Species
musclus
Sex
Age
Identity
Tissue for Primary Cancer
hemo-lymphocytic
Case history
lymphoid neoplasm
Metastasis
Tissue Metastasized
Genetics
Life Span
infinite
Crisis PDL
Morphology
lymphoblast-like
Character
lymphoid neoplasm, derived from P388
Classify
tumor
Established by
Dawe, C. J. & Potter, M.
Registered by
Iwasa,S.
Regulation for Distribution
Comment
Year
1985
Medium
RPMI1640 medium with 10% fetal bovine serum
Methods for Passages
dilution
Cell Number on Passage
Race
CO2 Conc.
5 %
Tissue Sampling
lymph node
Tissue Type
Detection of virus genome fragment by Real-time PCR
Detected DNA Virus
not tested
Detected RNA Virus
not tested
Images
LOT Information
Cell No.
IFO50036
Cell Name
P388D1
LOT No.
337
Lot Specification
distribution
Medium
RPMI 1640, 90% ; FBS, 10%
Temperature
37 C
Cell Density at Seeding
Methods for Passages
dilution
Doubling Time
Cell Number in Vial (cells/1ml)
1 x 10^6
Viability at cell freezing (%)
93
Antibiotics Used
free
Passage Number
Unknown (5 at IFO)
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
NP, MD, LD
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Exoteric Gene
Medium for Freezing
10% DMSO, 20% serum (final conc.) - culture medium
CO2 Conc.
Viability immediately after thawing (%)
Additional information
Cell No.
IFO50036
Cell Name
P388D1
LOT No.
338
Lot Specification
distribution
Medium
RPMI 1640, 90% ; FBS, 10%
Temperature
37 C
Cell Density at Seeding
Methods for Passages
dilution
Doubling Time
Cell Number in Vial (cells/1ml)
1 x 10^6
Viability at cell freezing (%)
93
Antibiotics Used
free
Passage Number
Unknown (5 at IFO)
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
NP, MD, LD
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Exoteric Gene
Medium for Freezing
10% DMSO, 20% serum (final conc.) - culture medium
CO2 Conc.
Viability immediately after thawing (%)
Additional information
Cell No.
IFO50036
Cell Name
P388D1
LOT No.
04102019
Lot Specification
distribution
Medium
RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012)
Temperature
37 C
Cell Density at Seeding
1.3 - 2.6 x 10^5 cells/mL
Methods for Passages
Simple dilution.
Doubling Time
approx. 25 hrs.
Cell Number in Vial (cells/1ml)
2.4 x 10^6
Viability at cell freezing (%)
94.5
Antibiotics Used
free
Passage Number
Unknown (7 at bank)
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
No
Exoteric Gene
Medium for Freezing
CO2 Conc.
5%
Viability immediately after thawing (%)
82.8
Additional information