IFO50040 Namalwa
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | IFO50040 | Cell Name | Namalwa |
|---|---|---|---|
| Profile | This cell line produce interferon. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | Age | ||
| Identity | available | Tissue for Primary Cancer | hemo-lymphocytic |
| Case history | Burkitt's lymphoma | Metastasis | |
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphoblast-like | Character | lymphoma, Burkitt's |
| Classify | tumor | Established by | Singh, S., (biopsy taken by) |
| Registered by | Takeuchi,M. | Regulation for Distribution | |
| Comment | Year | 1985 | |
| Medium | RPMI1640 medium with 10% fetal bovine serum | Methods for Passages | dilution |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5 % | Tissue Sampling | lymphoblastoid |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | + | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:464569 | Large-scale production and concentration of human lymphoid interferon. Klein F,Ricketts RT,Jones WI,DeArmon IA,Temple MJ,Zoon KC,Bridgen PJ Antimicrob Agents Chemother. 1979 Mar;15(3):420-7 |
| Pubmed id:4365093 | Sensitivity to EBV superinfection and IUdR inducibility of hybrid cells formed between a sensitive and a relatively resistant Burkitt lymphoma cell line. Nyormoi O,Klein G,Adams A,Dombos L Int J Cancer. 1973 Sep 15;12(2):396-408 |
| Pubmed id:4122458 | Sensitivity of Epstein-Barr virus (EBV) producer and non-producer human lymphoblastoid cell lines to superinfection with EB-virus. Klein G,Dombos L,Gothoskar B Int J Cancer. 1972 Jul 15;10(1):44-57 |
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LOT Information
| Cell No. | IFO50040 | Cell Name | |
|---|---|---|---|
| LOT No. | 07182008 | Lot Specification | distribution |
| Medium | RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099, lot 484375) | Temperature | 37 C |
| Cell Density at Seeding | 0.5 - 2 x 10^5 cells/mL | Methods for Passages | dilution (suspension culture) |
| Doubling Time | approx. 1 day | Cell Number in Vial (cells/1ml) | 8.8 x 10^6 |
| Viability at cell freezing (%) | 90.0 | Antibiotics Used | free |
| Passage Number | Unknown (6 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | D5S818:12,13 D13S317:11,12 D7S820:11 D16S539:9 VWA:14 TH01:7,9.3 AM:X TPOX:6,11 CSF1PO:10,11 |
|
| Adhesion | No | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - RPMI 1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | IFO50040 | Cell Name | Namalwa |
|---|---|---|---|
| LOT No. | 844 | Lot Specification | distribution |
| Medium | RPMI 1640, 90% ; FBS, 10% | Temperature | 37 C |
| Cell Density at Seeding | Methods for Passages | dilution | |
| Doubling Time | Cell Number in Vial (cells/1ml) | 1 x 10^7 | |
| Viability at cell freezing (%) | 88 | Antibiotics Used | free |
| Passage Number | Unknown (6 at IFO) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NP, G6PD(type B), MD |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | 10% DMSO, 20% serum (final conc.) - culture medium | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | IFO50040 | Cell Name | Namalwa |
|---|---|---|---|
| LOT No. | 04032019 | Lot Specification | distribution |
| Medium | RPMI-1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) | Temperature | 37 C |
| Cell Density at Seeding | 2 - 3 x 10^5 cells/mL | Methods for Passages | Simple dilution (suspension culture) |
| Doubling Time | approx. 17 hrs. | Cell Number in Vial (cells/1ml) | 8.5 x 10^6 |
| Viability at cell freezing (%) | 86.7 | Antibiotics Used | free |
| Passage Number | Unknown (6 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | No | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - RPMI1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 85.9 | Additional information |