IFO50069 Alexander cells
Cell information
Important Notice(s)The Alexander cells: another name of PLC/PRF/5 cell line.
Cell type：general cells (View Pricing Information)
| JCRB No. | IFO50069 | Cell Name | Alexander cells |
|---|---|---|---|
| Profile | hepatoma, HBs antigen-positive. This cell line is the same cell line as PLF/PRF/5 (other name of PLC/PRF/5). | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 24 |
| Identity | available | Tissue for Primary Cancer | liver, gallbladder |
| Case history | malignant liver cancer | Metastasis | No |
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | hepatoma, HBs antigen-positive |
| Classify | tumor | Established by | Alexander, J. J. |
| Registered by | Takeuchi,M. | Regulation for Distribution | |
| Comment | Year | 1985 | |
| Medium | RPMI1640 medium with 10% fetal bovine serum | Methods for Passages | trypsin-EDTA |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5 % | Tissue Sampling | liver |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | + | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:63998 | Establishment of a continuously growing cell line from primary carcinoma of the liver. Alexander JJ,Bey EM,Geddes EW,Lecatsas G S Afr Med J. 1976 Dec 18;50(54):2124-8 |
| Pubmed id:187208 | Hepatitis B surface antigen produced by a human hepatoma cell line. MacNab GM,Alexander JJ,Lecatsas G,Bey EM,Urbanowicz JM Br J Cancer. 1976 Nov;34(5):509-15 |
| Images |
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LOT Information
| Cell No. | IFO50069 | Cell Name | Alexander cells |
|---|---|---|---|
| LOT No. | 1011 | Lot Specification | distribution |
| Medium | RPMI 1640, 90% ; FBS, 10% | Temperature | 37 C |
| Cell Density at Seeding | Methods for Passages | trypsin-EDTA | |
| Doubling Time | Cell Number in Vial (cells/1ml) | 6 x 10^5 | |
| Viability at cell freezing (%) | 93 | Antibiotics Used | free |
| Passage Number | Unknown (6 at IFO) | PDL | |
| Sterility: MYCOPLASMA | + | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NP, G6PD(type A), MD |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | 10% DMSO, 20% serum (final conc.) - culture medium | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | IFO50069 | Cell Name | Alexander cells |
|---|---|---|---|
| LOT No. | 10252013 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10099). | Temperature | 37C |
| Cell Density at Seeding | 3.5 - 4.4 x 10^4 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin and 0.02% EDTA. |
| Doubling Time | Cell Number in Vial (cells/1ml) | 1.9 x 10^6 (cells/1 | |
| Viability at cell freezing (%) | Antibiotics Used | free | |
| Passage Number | Unknown (11 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | G6PD(typeA),NP,LDH were examined. Human |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | D5S818:12 D13S317:11,12 D7S820:9,11 D16S539:13 VWA:15,16 TH01:7,8 AM:X TPOX:8 CSF1PO:10 |
|
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | 5% | |
| Viability immediately after thawing (%) | 89.0 | Additional information |