IFO50075 WI-38

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50075 Cell Name   WI-38
Profile   Normal diploid fibroblast with limeted life span. This cell line at around 30 PDL is distributed. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   3-month
Identity   available Tissue for Primary Cancer   lung
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   finite Crisis PDL   40-60
Morphology   fibroblast-like Character   fetus lung normal diploid fibroblast
Classify   primary Established by   Hayflick, L.
Registered by   Takeuchi,M. Regulation for Distribution   
Comment    Year   1985
Medium   Basal medium Eagle with 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   lung
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0579
Reference
Pubmed id:4290661 SV40 transformation of human diploid cells. A parallel study of viral and karyologic parameters.
Girardi AJ,Weinstein D,Moorhead PS
Ann Med Exp Biol Fenn. 1966;44(2):242-54
Pubmed id:14315085 THE LIMITED IN VITRO LIFETIME OF HUMAN DIPLOID CELL STRAINS.
HAYFLICK L
Exp Cell Res. 1965 Mar;37():614-36
Pubmed id:14288316 SV40-INDUCED TRANFORMATION OF HUMAN DIPLOID CELLS: CRISIS AND RECOVERY.
GIRARDI AJ,JENSEN FC,KOPROWSKI H
J Cell Comp Physiol. 1965 Feb;65():69-83
Pubmed id:13905660 Preparation of poliovirus vaccines in a human fetal diploid cell strain.
HAYFLICK L,PLOTKIN SA,NORTON TW,KOPROWSKI H
Am J Hyg. 1962 Mar;75():240-58

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LOT Information

Cell No.   IFO50075 Cell Name   
LOT No.   04092010 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   approx. 4 - 5 x 10^4 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 1.5 days Cell Number in Vial (cells/1ml)   8.0 x 10^5
Viability at cell freezing (%)   92 Antibiotics Used   free
Passage Number   P19 PDL   33
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50075 Cell Name   
LOT No.   06102008 Lot Specification   distribution
Medium   Basal medium Eagle with 10% fetal bovine serum (GIBCO Cat.# 10099, lot 484375) Temperature   37 C
Cell Density at Seeding   3 - 5 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA (37 C for 5 min). Split ratio = 1:4 - 1:6
Doubling Time   approx. 1.5 days Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   86.9 Antibiotics Used   free
Passage Number   P19 PDL   32
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - BME CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50075 Cell Name   
LOT No.   03202006 Lot Specification   distribution
Medium   Basal medium Eagle's with 10% fetal bovine serum (FBS lot GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   3 - 4 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 2 days Cell Number in Vial (cells/1ml)   6.9 x 10^5
Viability at cell freezing (%)   92.6 Antibiotics Used   free
Passage Number   P19 PDL   31
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - BME CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50075 Cell Name   
LOT No.   06012001 Lot Specification   distribution
Medium   Basal medium Eagle's with 10% fetal bovine serum Temperature   37 C
Cell Density at Seeding   5 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS
Doubling Time    Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)   85 Antibiotics Used   free
Passage Number   P19 PDL   31
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50075 Cell Name   WI-38
LOT No.   1074 Lot Specification   distribution
Medium   Eagle's basal medium with Hanks' salts, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   8 x 10^5
Viability at cell freezing (%)   87 Antibiotics Used   free
Passage Number   P20 PDL   32
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD(type B), AST
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50075 Cell Name   WI-38
LOT No.   09022013 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10099) Temperature   37C
Cell Density at Seeding   1.8 - 6.5 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   1.4 x 10^6 (cells/1m
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   P19 PDL   33.6
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB),NP,LDH were examined. Human,not HeLa
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   87.2%
Additional information   
Cell No.   IFO50075 Cell Name   WI-38
LOT No.   09292017 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   approx. 6 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 35 hrs. Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)   80 Antibiotics Used   free
Passage Number   P19 PDL   31.9
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   80
Additional information   
Cell No.   IFO50075 Cell Name   WI-38
LOT No.   11212019 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   2.5 - 5.2 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 36 hrs. Cell Number in Vial (cells/1ml)   1.8 x 10^6
Viability at cell freezing (%)   98 Antibiotics Used   free
Passage Number   P20 PDL   32.7
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   88
Additional information   
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