IFO50077 IT-45R91 Cell information
Cell type:general cells
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JCRB No.
IFO50077
Cell Name
IT-45R91
Profile
thymic fibroblast, (Wistar rat)
Other Name
Animal
rat
Strain
Wistar
Genus
Rattus
Species
norvegicus
Sex
Age
Identity
Tissue for Primary Cancer
thymus
Case history
Metastasis
Tissue Metastasized
Genetics
Life Span
infinite
Crisis PDL
Morphology
fibroblast-like
Character
thymic fibroblast
Classify
Established by
Itoh, T.
Registered by
Itoh,T.
Regulation for Distribution
Comment
Year
1985
Medium
Eagle's minimal essential medium with 10% fetal bovine serum
Methods for Passages
trypsin (0.05%), subcultivation ratio ; 1:10
Cell Number on Passage
Race
CO2 Conc.
5 %
Tissue Sampling
thymus
Tissue Type
Detection of virus genome fragment by Real-time PCR
Detected DNA Virus
not tested
Detected RNA Virus
not tested
Images
LOT Information
Cell No.
IFO50077
Cell Name
IT-45R91
LOT No.
976
Lot Specification
distribution
Medium
E.MEM, 90% ; FBS, 10%
Temperature
37 C
Cell Density at Seeding
Methods for Passages
trypsin (0.05%), subcultivation ratio ; 1:10
Doubling Time
Cell Number in Vial (cells/1ml)
2 x 10^6
Viability at cell freezing (%)
88
Antibiotics Used
free
Passage Number
Unknown (11 at IFO)
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
NP, G6PD, MD
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Exoteric Gene
Medium for Freezing
10% DMSO, 20% serum (final conc.) - culture medium
CO2 Conc.
Viability immediately after thawing (%)
Additional information