IFO50079 Flow7000

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50079 Cell Name   Flow7000
Profile   Normal diploid fibroblasts with limited life span. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   14-week fetus
Identity   available Tissue for Primary Cancer   skin
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   finite Crisis PDL   
Morphology   fibroblast-like Character   skin normal diploid fibroblast
Classify   primary Established by   
Registered by   Sasada,R. Regulation for Distribution   
Comment    Year   1985
Medium   Eagle's minimal essential medium with 10% fetal bovine serum Methods for Passages   trypsin-EDTA, subcultivation ratio =1/2
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   prepuce
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus tested
HCV - HTLV-1 -
HIV-1 - HTLV-2 -
HIV-2 - HAV -
-/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2904
Reference
Pubmed id:841332 Enzyme polymorphisms as genetic signatures in human cell cultures.
O'Brien SU,Kleiner G,Olson R,Shannon JE
Science. 1977 Mar 25;195(4284):1345-8

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LOT Information

Cell No.   IFO50079 Cell Name   
LOT No.   04092004 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum ( FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   2-3 x 10^4 cells/sq.cm Methods for Passages   0.25% trypsin and 0.02% EDTA. This lot of cells can grow at least additional 3 passages at 1/4 split.
Doubling Time   ca. 3 days Cell Number in Vial (cells/1ml)   7.5 x 10^5
Viability at cell freezing (%)   93.8 Antibiotics Used   free
Passage Number   9 at bank PDL   +10.4
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type A), AST and LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50079 Cell Name   Flow7000
LOT No.   335 Lot Specification   distribution
Medium   E.MEM, 90% + FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA, subcultivation ratio ; 1:2
Doubling Time    Cell Number in Vial (cells/1ml)   2 x 10^6
Viability at cell freezing (%)   65 Antibiotics Used   free
Passage Number   Unknown (5 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, MD, LD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:9,13
D13S317:12
D7S820:9,12
D16S539:10,12
VWA:15,17
TH01:8
AM:X
TPOX:8,11
CSF1PO:9,11
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   5 %
RFLP   HeLa patterns by STR-PCR experiments observed in this lot of cells after several passages. Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   IFO50079 Cell Name   Flow7000
LOT No.   10132017 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   0.3 - 1.1 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 42 hrs. Cell Number in Vial (cells/1ml)   6.4 x 10^5
Viability at cell freezing (%)   89.3 Antibiotics Used   free
Passage Number   Unknown (9 at bank) PDL   +11
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   89.3
Additional information   
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