IFO50081 Neuro-2a

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50081 Cell Name   Neuro-2a
Profile   Derivative of C1300 tumor. C1300 tumor was originally described as a spontaneous tumor of the region of spinal cord. Later studies suggested that it is drived from sympathetic cells in peripheral nervous system. Other Name   
Animal   mouse Strain   A albino
Genus   Mus Species   musclus
Sex    Age   
Identity    Tissue for Primary Cancer   neural
Case history   neuroblastoma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   neuron & amoeboid-L Character   neuroblastoma, Td = 20 hrs.
Classify   tumor Established by   Klebe, R. J. & Ruddle, F. H.
Registered by   Suno,M. Regulation for Distribution   
Comment    Year   1985
Medium   Eagle's minimal essential medium with non-essential amino acids and 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage   split ratio = 1/6 - 1/20. Race   
CO2 Conc.   5 % Tissue Sampling   region of spinal cord
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0470
Reference
Pubmed id:5263744Isolation of microtubule protein from cultured mouse neuroblastoma cells.
Olmsted JB,Carlson K,Klebe R,Ruddle F,Rosenbaum J
Proc Natl Acad Sci U S A. 1970 Jan;65(1):129-36

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LOT Information

Cell No.   IFO50081 Cell Name   
LOT No.   08182011 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non essential amino acids (0.1 mM) and 10% fetal bovine serum (FBS: GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   approx. 1.0 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Passage twice a week.
Doubling Time   approx. 27 hrs. Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)   94.4 Antibiotics Used   free
Passage Number   P181 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM with NEAA CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50081 Cell Name   
LOT No.   03072008 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids and 10% fetal bovine serum (GIBCO Cat.# 10099, lot 484375) Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/ml (subculture twice a week Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 20 hrs Cell Number in Vial (cells/1ml)   4.4 x 10^6
Viability at cell freezing (%)   97.9 Antibiotics Used   free
Passage Number   P182 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50081 Cell Name   
LOT No.   10212004 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essential amino acids and 10% fetal bovine serum (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   3 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA, subcultivate twice a week.
Doubling Time   16 hrs Cell Number in Vial (cells/1ml)   2.0 x 10^6
Viability at cell freezing (%)   97.6 Antibiotics Used   free
Passage Number   P181 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50081 Cell Name   
LOT No.   12132002 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essential amino acids and 10% fetal bovine serum (FBS lot; BioWhittaker 0S069F) Temperature   37 C
Cell Density at Seeding   0.5 - 1 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   2.5 x 10^6
Viability at cell freezing (%)   85 Antibiotics Used   free
Passage Number   P182 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS-culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50081 Cell Name   
LOT No.   593 Lot Specification   distribution
Medium   E.MEM with NEAA, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number    PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50081 Cell Name   Neuro-2a
LOT No.   09262014 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids and 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   6.6 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   2.1 x 10^6
Viability at cell freezing (%)   98 Antibiotics Used   free
Passage Number   P180 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   98 Additional information   
Cell No.   IFO50081 Cell Name   Neuro-2a
LOT No.   12142015 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids and 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   0.8 - 1.1 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 40 - 42 hrs Cell Number in Vial (cells/1ml)   2.3 x 10^6
Viability at cell freezing (%)   92.0 Antibiotics Used   free
Passage Number   P181 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM with NEAA CO2 Conc.   5%
Viability immediately after thawing (%)   92.0 Additional information   
Cell No.   IFO50081 Cell Name   Neuro-2a
LOT No.   03152018 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids and 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   1.6 x 10^6
Viability at cell freezing (%)   90.9 Antibiotics Used   free
Passage Number   P182 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   90.9 Additional information   
Cell No.   IFO50081 Cell Name   Neuro-2a
LOT No.   09112020 Lot Specification   distribution
Medium   Eagle's minimum essential medium(GIBCO) with non essential amino acid (GIBCO cat.No.11140-050) and 10% heat inactivated fetal bovine serum(SIGMA,Cat#172012,Lot#12J396) Temperature   37 C
Cell Density at Seeding   0.5-3.7x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.(Split ratio=1/6-1/20)
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.0x10^6
Viability at cell freezing (%)   97.00 Antibiotics Used   free
Passage Number   p181 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   64.7 Additional information   
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Cell No.   IFO50081 Cell Name   Neuro-2a
LOT No.   05302023 Lot Specification   distribution
Medium   MEM with Earle's Salts, L-Gln and Non-Essential Amino Acids (Nacalai Tesque #21443-15) and 10% heat-inactivated fetal bovine serum (Nichirei Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   0.76 - 1.5 x 10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 1 mM EDTA .
Doubling Time    Cell Number in Vial (cells/1ml)   1.95 x 10^6
Viability at cell freezing (%)   95.2 Antibiotics Used   free
Passage Number   P182 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER (LYMPHOTEC Inc.,CS-02-001, NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   25.1 Additional information   
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