IFO50090 4G12 hybridoma

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50090 Cell Name   4G12 hybridoma
Profile   This cell line produces human monoclonal 4G12 antibody (Ig lambda). The 4G12 antibody recognizes a glycoprotein with Mr = 65,000. Immunohistochemically, 4G12 reacts especially with lung carcinomas and with some malignant tumors. Other Name   
Animal   mouse x human Strain   
Genus    Species   
Sex    Age   
Identity    Tissue for Primary Cancer   antibody-producing hybridoma
Case history    Metastasis   
Tissue Metastasized    Genetics   human lymphoid x mouse myeloma (P3U1)
Life Span   infinite Crisis PDL   
Morphology   round Character   4G12 antibody, preferentially reacting with lung carcinoma-associated antigen
Classify    Established by   Taniguchi, M.
Registered by   Matushiro,Y. Regulation for Distribution   
Comment    Year   1986
Medium   RPMI1640 medium with 4% fetal bovine serum Methods for Passages   gentle pipetting and dilution
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   
Tissue Type   hybridoma, lymphoid x myeloma

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Reference
Pubmed id:9177469Establishment of internal-image anti-idiotype monoclonal antibodies to a human antibody to lung cancer.
Saito H,Taniguchi M,Fukasawa T,Yamaguchi Y,Fujisawa T
Cancer Immunol Immunother. 1997 Apr;44(2):83-7
Pubmed id:2455061Characterization of a human monoclonal antibody with broad reactivity to malignant tumor cells.
Saito H,Uchiyama K,Nakamura I,Hiraoka H,Yamaguchi Y,Taniguchi M
J Natl Cancer Inst. 1988 Jul 20;80(10):728-34

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LOT Information

Cell No.   IFO50090 Cell Name   4G12 hybridoma
LOT No.   06232004 Lot Specification   distribution
Medium   RPMI1640 medium with 4% fetal bovine serum(GIBCO 3762782S). Temperature   37 C
Cell Density at Seeding   0.5-1.0 x 10^5 cells/ml Methods for Passages   Pipetting or trypsin treatment. A portion of cells strongly adhere to substratum, and is hardly to detach by pipetting.
Doubling Time   1.2 days. Cell Number in Vial (cells/1ml)   1.6 x 10^6
Viability at cell freezing (%)   92.8 Antibiotics Used   Free. Pretreated with MC210 in this lot only.
Passage Number   P6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD, NP, MD. Human-mouse hybrid confirmed.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   IFO50090 Cell Name   4G12 hybridoma
LOT No.   1020 Lot Specification   distribution
Medium   RPMI 1640, 96% ; FBS, 4% Temperature   37 C
Cell Density at Seeding    Methods for Passages   dilution
Doubling Time    Cell Number in Vial (cells/1ml)   5 x 10^5
Viability at cell freezing (%)   75 Antibiotics Used   free
Passage Number   Unknown (>3 at IFO) PDL   
Sterility: MYCOPLASMA   + Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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