IFO50098 F2408-No.3

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50098 Cell Name   F2408-No.3
Profile   HGPRT-deficient line from F2408 (IFO 50015). Other Name   
Animal   rat Strain   Fischer
Genus   Rattus Species   norvegicus
Sex    Age   embryo
Identity    Tissue for Primary Cancer   embryo
Case history    Metastasis   
Tissue Metastasized    Genetics   HGPRT-deficient
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   HGPRT-deficient F2408 cell line
Classify   mutant Established by   Hakura, A.
Registered by   Hakura,A. Regulation for Distribution   
Comment    Year   1986
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   embryonic fibroblast
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_L071
Reference
Pubmed id:6829165Rat mutant cells showing temperature sensitivity for transformation by wild-type Moloney murine sarcoma virus.
Inoue H,Yutsudo M,Hakura A
Virology. 1983 Feb;125(1):242-5

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LOT Information

Cell No.   IFO50098 Cell Name   F2408-No.3
LOT No.   1146 Lot Specification   distribution
Medium   D.MEM, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding   0.5-1.0 x 10^5 cells/ml Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   2.2 x 10^6
Viability at cell freezing (%)   87 Antibiotics Used   free
Passage Number   Unknown (>3 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   MD, G6PD, LD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.)-culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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