IFO50105 GH3

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50105 Cell Name   GH3
Profile   Rat pituitary tumor secreting somatotrophin. Other Name   
Animal   rat Strain   Wistar-Furth
Genus   Rattus Species   norvegicus
Sex   F Age   7-month-old
Identity    Tissue for Primary Cancer   pituitary
Case history   pituitary tumor Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   pituitary tumor, somatotrophin-secreting
Classify   tumor Established by   Tashjian Jr., A. H. et al.
Registered by   Shiota,K. Regulation for Distribution   
Comment    Year   1986
Medium   Ham's F10 medium with 15% horse serum and 2.5% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   pituitary gland
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0273
Reference
Pubmed id:5541958Growth in suspension culture of rat pituitary cells which produce growth hormone and prolactin.
Bancroft FC,Tashjian AH Jr
Exp Cell Res. 1971 Jan;64(1):125-8
Pubmed id:5389137Control of growth hormone production by a clonal strain of rat pituitary cells. Stimulation by hydrocortisone.
Bancroft FC,Levine L,Tashjian AH Jr
J Cell Biol. 1969 Dec;43(3):432-41
Pubmed id:4951281Establishment of clonal strains of rat pituitary tumor cells that secrete growth hormone.
Tashjian AH Jr,Yasumura Y,Levine L,Sato GH,Parker ML
Endocrinology. 1968 Feb;82(2):342-52

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LOT Information

Cell No.   IFO50105 Cell Name   GH3
LOT No.   456 Lot Specification   distribution
Medium   Ham's F10, 82.5% ; horse serum, 15% ; FBS, 2.5% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   2 x 10^6
Viability at cell freezing (%)   93 Antibiotics Used   free
Passage Number   P21 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD, MD, AST
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50105 Cell Name   GH3
LOT No.   09152016 Lot Specification   distribution
Medium   Ham's F10 medium with 15% horse serum and 2.5% fetal bovine serum Temperature   37 C
Cell Density at Seeding   2.1 - 5.1 x 10^5 cells/mL Methods for Passages   0.25% trypsin and 0.02% EDTA. Cell attachment is weak, and some cells will pile up and grow as suspension.
Doubling Time   48 - 113 hrs Cell Number in Vial (cells/1ml)   2.3 x 10^6
Viability at cell freezing (%)   87.8 Antibiotics Used   free
Passage Number   P23 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO in culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   87.8 Additional information   
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