IFO50106 HOS

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50106 Cell Name   HOS
Profile   osteosarcoma Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   13
Identity   available Tissue for Primary Cancer   sarcoma
Case history   osteogenic sarcoma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   polygonal Character   osteosarcoma
Classify   tumor Established by   Rhim, J. S. et al.
Registered by   Igarashi,K. Regulation for Distribution   
Comment    Year   1986
Medium   Eagle's minimal essential medium with non-essential amino acids and 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   bone
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0312
Reference
Pubmed id:265298 Characterization of human cells transformed in vitro by N-methyl-N'-nitro-N-nitrosoguanidine.
Rhim JS,Putman DL,Arnstein P,Huebner RJ,McAllister RM
Int J Cancer. 1977 Apr 15;19(4):505-10
Pubmed id:165148 Non-producer human cells induced by murine sarcoma virus.
Rhim JS,Cho HY,Huebner RJ
Int J Cancer. 1975 Jan 15;15(1):23-9
Pubmed id:171229 Characterization of non-producer human cells induced by Kirsten sarcoma virus.
Rhim JS,Cho HY,Vernon ML,Arnstein P,Huebner RJ,Gilden RV
Int J Cancer. 1975 Nov 15;16(5):840-9
Pubmed id:5100401 Cultivation in vitro of cells derived from a human osteosarcoma.
McAllister RM,Gardner MB,Greene AE,Bradt C,Nichols WW,Landing BH
Cancer. 1971 Feb;27(2):397-402

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LOT Information

Cell No.   IFO50106 Cell Name   
LOT No.   04192002 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essential amino acids and 10% fetal bovine serum Temperature   37 C
Cell Density at Seeding   2 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   D.T. = ca 20 hrs Cell Number in Vial (cells/1ml)   1.3 x 10^6
Viability at cell freezing (%)   90.7 Antibiotics Used   free
Passage Number   P6* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS-culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50106 Cell Name   HOS
LOT No.   450 Lot Specification   distribution
Medium   E.MEM with NEAA, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   7 x 10^5
Viability at cell freezing (%)   92 Antibiotics Used   free
Passage Number   Unknown (6 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD(type B), MD, PepB
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50106 Cell Name   HOS
LOT No.   02272015 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids and 10% fetal bovine serum (FBS;GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   0.9 - 2.8 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 21 hrs Cell Number in Vial (cells/1ml)   2.7 x 10^6
Viability at cell freezing (%)   95.5 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:13
D13S317:12
D7S820:11,12
D16S539:10,13
VWA:18
TH01:6
AM:X
TPOX:8,11
CSF1PO:12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   95.5
Additional information   
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