IFO50110 C6

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50110 Cell Name   C6
Profile   glial tumor, GFAP-positive Other Name   
Animal   rat Strain   
Genus   Rattus Species   norvegicus
Sex    Age   
Identity    Tissue for Primary Cancer   neural
Case history   glial tumor Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   glial tumor, GFAP-positive
Classify   tumor Established by   Benda, P. et al.
Registered by   Suno,M. Regulation for Distribution   
Comment    Year   1986
Medium   Ham's F10 medium with 2.5% fetal bovine serum and 10% horse serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   brain
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0194
Reference
Pubmed id:4873531Differentiated rat glial cell strain in tissue culture.
Benda P,Lightbody J,Sato G,Levine L,Sweet W
Science. 1968 Jul 26;161(3839):370-1

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LOT Information

Cell No.   IFO50110 Cell Name   
LOT No.   07242006 Lot Specification   distribution
Medium   Ham's F10 medium with 2.5% fetal bovine serum and 10% horse seum Temperature   37 C
Cell Density at Seeding   1-3 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   2.4 x 10^6
Viability at cell freezing (%)   97.9 Antibiotics Used   free
Passage Number   P61 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as rat by NP, G6PD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Ham's F10 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50110 Cell Name   C6
LOT No.   993 Lot Specification   distribution
Medium   Ham's F10, 87.5% ; FBS, 2.5% ; horse serum, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   2 x 10^6
Viability at cell freezing (%)   90 Antibiotics Used   free
Passage Number   P62 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD, PepB
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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