IFO50111 GRSL

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50111 Cell Name   GRSL
Profile   thymic leukemia, (GR/A mouse) Other Name   
Animal   mouse Strain   GRS/A
Genus   Mus Species   musclus
Sex    Age   
Identity    Tissue for Primary Cancer   hemo-lymphocytic
Case history   thymic leukemia Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphoblast-like Character   thymic leukemia
Classify   tumor Established by   Hilgers, J.
Registered by   Takeuchi,M. Regulation for Distribution   
Comment   This line was cured of mycoplasmal contamination by antibiotic-treatment at the IFO. Year   1986
Medium   RPMI1640 medium with 10% fetal bovine serum Methods for Passages   dilution
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   thymus
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_5509
Reference
Pubmed id:3401476Effect of dietary lipids on plasma lipoproteins and fluidity of lymphoid cell membranes in normal and leukemic mice.
Damen J,De Widt J,Hilkmann H,Van Blitterswijk WJ
Biochim Biophys Acta. 1988 Aug 18;943(2):166-74
Pubmed id:204164Interaction between viral and genetic factors in murine mammary cancer.
Hilgers J,Bentvelzen P
Adv Cancer Res. 1978;26():143-95

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LOT Information

Cell No.   IFO50111 Cell Name   GRSL
LOT No.   457 Lot Specification   distribution
Medium   RPMI 1640, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   dilution
Doubling Time    Cell Number in Vial (cells/1ml)   7 x 10^5
Viability at cell freezing (%)   88 Antibiotics Used   free
Passage Number   Unknown (7 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD, MD, AST
Chromosome Mode   41 Chromosome Information   38-84
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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