IFO50151 MEG-01

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50151 Cell Name   MEG-01
Profile   Contains Ph1 chromosome. Expresses megakaryoblastic markers as GPIIb/IIIa, GPIb, FVIII-related antigens, and also CD9, CDw14. It is reported that MEG-01 cells produced platelet-like particles. Morphological changes by phorbol ester. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   56
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   cronic leukemia Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   Myeloblast-like Character   leukemia, chronic megakaryoblastic
Classify   tumor Established by   Ogura, M. et al.
Registered by   Ogura,M. Regulation for Distribution   
Comment    Year   1987
Medium   RPMI1640 medium with 20% fetal bovine serum Methods for Passages   dilution
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   bone marrow
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0425
Reference
Pubmed id:2998511Establishment of a novel human megakaryoblastic leukemia cell line, MEG-01, with positive Philadelphia chromosome.
Ogura M,Morishima Y,Ohno R,Kato Y,Hirabayashi N,Nagura H,Saito H
Blood. 1985 Dec;66(6):1384-92

Images



LOT Information

Cell No.   IFO50151 Cell Name   MEG-01
LOT No.   10292004 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   5 - 10 x 10^4 cells/ml Methods for Passages   Cells were harvested by pipetting and diluted to fresh medium. (Some cells could not be detached by pipetting.)
Doubling Time   ca. 54 hrs Cell Number in Vial (cells/1ml)   2.2 x 10^6
Viability at cell freezing (%)   96 Antibiotics Used   free
Passage Number   P7 at cellbank PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD, MD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   IFO50151 Cell Name   MEG-01
LOT No.   968 Lot Specification   distribution
Medium   RPMI 1640, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   dilution
Doubling Time    Cell Number in Vial (cells/1ml)   8 x 10^5
Viability at cell freezing (%)   85 Antibiotics Used   free
Passage Number   Unknown (7 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD(type B), AST
Chromosome Mode   104 Chromosome Information   71-193
Surface Antigen   Gp IIb/IIIa DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50151 Cell Name   MEG-01
LOT No.   679 Lot Specification   distribution
Medium   RPMI 1640, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   dilution
Doubling Time    Cell Number in Vial (cells/1ml)   3 x 10^6
Viability at cell freezing (%)   86 Antibiotics Used   free
Passage Number   Unknown (11 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD(type B), MD
Chromosome Mode   104 Chromosome Information   71-193
Surface Antigen   Gp IIb/IIIa DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50151 Cell Name   MEG-01
LOT No.   458 Lot Specification   distribution
Medium   RPMI 1640, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   dilution
Doubling Time    Cell Number in Vial (cells/1ml)   7 x 10^5
Viability at cell freezing (%)   93 Antibiotics Used   free
Passage Number   Unknown (6 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD(type B), MD
Chromosome Mode   104 Chromosome Information   71-193
Surface Antigen   Gp IIb/IIIa DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50151 Cell Name   MEG-01
LOT No.   02212013 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum Temperature   37 C
Cell Density at Seeding   2 - 3 x 10^5 cells / mL Methods for Passages   Dilution (suspension culture). Some adherent cells were detached by pipetting.
Doubling Time    Cell Number in Vial (cells/1ml)   4.8 x 10^5 cells / m
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   p6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   10% DMSO, 20% FBS, RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50151 Cell Name   MEG-01
LOT No.   08152017 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   0.5 - 1.1 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture). Some adherent cells were detached by pipetting.
Doubling Time   approx. 34 hrs. Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)   above 99 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   above 99 Additional information   
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