IFO50154 FBHE Cell information
Cell type：general cells
(View Pricing Information )
JCRB No.
IFO50154
Cell Name
FBHE
Profile
Normal diploid endothelial cells with limited life span. FGF-dependent for survival.
Other Name
Animal
bovine
Strain
Genus
Bos
Species
taurus
Sex
Age
fetus
Identity
Tissue for Primary Cancer
vascular system
Case history
endothelial cell
Metastasis
Tissue Metastasized
Genetics
Life Span
finite
Crisis PDL
Morphology
endothelial
Character
heart endothelial cell, FGF-dependent for survival
Classify
primary
Established by
Gospodarowicz, D.
Registered by
Sakaguchi,M.
Regulation for Distribution
Comment
Year
1987
Medium
Dulbecco's modified Eagle's medium with 10% fetal bovine serum and 2 ng/ml recombinant human basic FGF
Methods for Passages
0.05% trypsin-EDTA
Cell Number on Passage
Race
CO2 Conc.
5 %
Tissue Sampling
heart
Tissue Type
Detection of virus genome fragment by Real-time PCR
Detected DNA Virus
not tested
Detected RNA Virus
not tested
Images
LOT Information
Cell No.
IFO50154
Cell Name
FBHE
LOT No.
438
Lot Specification
distribution
Medium
D.MEM, 90% ; FBS, 10% ; rhbFGF, 2ng/ml
Temperature
37 C
Cell Density at Seeding
Methods for Passages
0.05% trypsin-EDTA
Doubling Time
Cell Number in Vial (cells/1ml)
2 x 10^6
Viability at cell freezing (%)
89
Antibiotics Used
free
Passage Number
P15
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
NP,PEPB
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Exoteric Gene
Medium for Freezing
10% DMSO, 20% serum (final conc.) - culture medium
CO2 Conc.
Viability immediately after thawing (%)
Additional information
