IFO50161 KT-5 Cell information
Cell type：general cells
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JCRB No.
IFO50161
Cell Name
KT-5
Profile
GFAP and S-100-positive, MBP, thy-1 and neurofilament-negative. (Tested at the IFO.).
Other Name
Animal
mouse
Strain
C3H
Genus
Mus
Species
musclus
Sex
Age
Identity
Tissue for Primary Cancer
neural
Case history
brain tumor
Metastasis
Tissue Metastasized
Genetics
Life Span
infinite
Crisis PDL
Morphology
astrocyte-like
Character
astrocyte cell line
Classify
tumor
Established by
Takeuchi, M.
Registered by
Takeuchi,M.
Regulation for Distribution
Comment
Year
1988
Medium
Ham's F12 medium with 4 mM HEPES and 10% fetal bovine serum (pH 7.3)
Methods for Passages
0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS, or 500 units/ml dispase in culture medium
Cell Number on Passage
Race
CO2 Conc.
5 %
Tissue Sampling
brain
Tissue Type
Detection of virus genome fragment by Real-time PCR
Detected DNA Virus
not tested
Detected RNA Virus
not tested
Images
LOT Information
Cell No.
IFO50161
Cell Name
LOT No.
03242005
Lot Specification
distribution
Medium
Ham's F12 medium with 4 mM HEPES and 10% fetal bovine serum (FBS lot; GIBCO 4218333S)
Temperature
37 C
Cell Density at Seeding
2-4 x 10^4 cells/ml
Methods for Passages
0.25% trypsin and 0.02% EDTA
Doubling Time
approx. 7 days
Cell Number in Vial (cells/1ml)
1.3 x 106
Viability at cell freezing (%)
95
Antibiotics Used
free
Passage Number
P23
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Yes
Exoteric Gene
Medium for Freezing
10% DMSO, 20% FBS - F12
CO2 Conc.
5%
Viability immediately after thawing (%)
Additional information
Cell No.
IFO50161
Cell Name
KT-5
LOT No.
488
Lot Specification
seed/distribution
Medium
Ham's F12 with 4 mM HEPES, 90% ; FBS, 10% (pH 7.3)
Temperature
37 C
Cell Density at Seeding
Methods for Passages
trypsin-EDTA
Doubling Time
Cell Number in Vial (cells/1ml)
Viability at cell freezing (%)
Antibiotics Used
free
Passage Number
PDL
Sterility: MYCOPLASMA
NT
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Exoteric Gene
Medium for Freezing
10% DMSO, 20% serum (final conc.) - culture medium
CO2 Conc.
Viability immediately after thawing (%)
Additional information
Cell No.
IFO50161
Cell Name
KT-5
LOT No.
07072014
Lot Specification
distribution
Medium
Ham's F12 medium with 4 mM HEPES and 10% fetal bovine serum (FBS; GIBCO Cat. # 10091).
Temperature
37 C
Cell Density at Seeding
1.2 x 10^6
Methods for Passages
Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time
Cell Number in Vial (cells/1ml)
1.2 x 10^6
Viability at cell freezing (%)
98
Antibiotics Used
free
Passage Number
P20
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Yes
Exoteric Gene
Medium for Freezing
10% DMSO, 20% FBS - Ham's F12
CO2 Conc.
5%
Viability immediately after thawing (%)
98
Additional information
Cell No.
IFO50161
Cell Name
KT-5
LOT No.
04182023
Lot Specification
distribution
Medium
Ham's F-12 medium with 4 mM HEPES and 10% fetal bovine serum (FBS; GIBCO Cat. # 10437).
Temperature
37 C
Cell Density at Seeding
4.2 - 6.6 x 10^4 cells/mL
Methods for Passages
Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time
approx. 43 hrs.
Cell Number in Vial (cells/1ml)
1.7 x 10^6
Viability at cell freezing (%)
96
Antibiotics Used
free
Passage Number
Unknown (20 at bank)
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Yes
Exoteric Gene
Medium for Freezing
10% DMSO, 20% FBS - basal culture medium
CO2 Conc.
5%
Viability immediately after thawing (%)
96
Additional information
