IFO50248 IT-79MTNC3

Cell information

Important Notice(s)

Dispaseの単位について

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50248 Cell Name   IT-79MTNC3
Profile   thymic nurse cell, (BALB/c mouse) Other Name   
Animal   mouse Strain   BALB/c
Genus   Mus Species   musclus
Sex   F Age   adult
Identity    Tissue for Primary Cancer   thymus
Case history   thymic tumor Metastasis   
Tissue Metastasized    Genetics   
Life Span   finite Crisis PDL   
Morphology   fibroblast-like Character   thymic nurse cell
Classify    Established by   Itoh, T.
Registered by   Itoh,T. Regulation for Distribution   
Comment    Year   1990
Medium   Dulbecco's modified Eagle's medium with Na-pyruvate and 10% fetal bovine serum Methods for Passages   dispase (500 units/ml) in culture medium.
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   thymus
Tissue Type   thymic nurse cell

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_U233
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LOT Information

Cell No.   IFO50248 Cell Name   IT-79MTNC3
LOT No.   636 Lot Specification   seed/distribution
Medium   D.MEM with Na-pyruvate, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   dispase (500 Units/ml)
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   Unknown PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD, MD, MPI, PepB, AST, LD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   Labeled as "NURSE R628F636" on glass vials.
Cell No.   IFO50248 Cell Name   IT-79MTNC3
LOT No.   10032017 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   5.5 - 7.5 x 10^4 cells/mL Methods for Passages   0.25% trypsin and 0.02% EDTA (originally dispase was used, but was turned out to be hard to detach cells)
Doubling Time   approx. 36 hrs. Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)   81.5 Antibiotics Used   free
Passage Number   Unknown (4 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DSMO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   81.5 Additional information   原著論文とシード作製時には継代にDispaseを使用しているが、本ロットではトリプシン処理に変更している。Dispaseでは剥離しにくい。トリプシン処理で培養に問題は生じない。
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