IFO50271 HUV-EC-C

Cell information

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IFO50271 HUV-EC-Cのウイルス検査成績について

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50271 Cell Name   HUV-EC-C
Profile   Normal endothelial cells with limited life span. For cultivation, endothelial cell growth supplement can be substituted by ca 10 ng/ml basic FGF. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   vascular system
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   finite Crisis PDL   50-60
Morphology   endothelial Character   normal endothelial cell, umbilical cord
Classify   primary Established by   Hoshi, H.
Registered by   Suzuki,N. Regulation for Distribution   
Comment    Year   1990
Medium   MCDB 107 with 10% FBS, 100ug/ml heparin and 50ug/ml endothelial cell growth supplement Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   umbilical cord, vein
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 + HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2959
Reference
Pubmed id:28755030 The human vascular endothelial cell line HUV-EC-C harbors the integrated HHV-6B genome which remains stable in long term culture.
Shioda S,Kasai F,Ozawa M,Hirayama N,Satoh M,Kameoka Y,Watanabe K,Shimizu N,Tang H,Mori Y,Kohara A
Cytotechnology. 2018 Feb;70(1):141-152
Pubmed id:8999915 Characterization of the binding of serum amyloid P to laminin.
Zahedi K
J Biol Chem. 1997 Jan 24;272(4):2143-8
Pubmed id:8621443 Characterization of novel vascular endothelial growth factor (VEGF) receptors on tumor cells that bind VEGF165 via its exon 7-encoded domain.
Soker S,Fidder H,Neufeld G,Klagsbrun M
J Biol Chem. 1996 Mar 8;271(10):5761-7
Pubmed id:6333682 Brain- and liver cell-derived factors are required for growth of human endothelial cells in serum-free culture.
Hoshi H,McKeehan WL
Proc Natl Acad Sci U S A. 1984 Oct;81(20):6413-7

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LOT Information

Cell No.   IFO50271 Cell Name   
LOT No.   05282012 Lot Specification   distribution
Medium   Ham's F12K medium with 10% FBS, 100 ug/ml heparin, 50 ug/ml endothelial cell growth supplement (ECGS). ECGS can be substituted by 10 ng/ml bFGF (FGF-2). Temperature   37C
Cell Density at Seeding   3 - 6 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 39 hrs Cell Number in Vial (cells/1ml)   7.9 x 10^5
Viability at cell freezing (%)   97.5 Antibiotics Used   free
Passage Number   P25 PDL   +13.3
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50271 Cell Name   
LOT No.   05232011 Lot Specification   distribution
Medium   Ham's F12K medium with 10% FBS, 100 ug/ml heparin, 50 ug/ml endothelial cell growth supplement (ECGS). ECGS can be substituted by 10 ng/ml bFGF (FGF-2). Temperature   37 C
Cell Density at Seeding   4.0 - 6.4 x 10^4 cells/mL Methods for Passages   0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS.
Doubling Time   approx. 2 days Cell Number in Vial (cells/1ml)   1.0 x 10^6
Viability at cell freezing (%)   96.2 Antibiotics Used   free
Passage Number   P25 PDL   +13
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50271 Cell Name   
LOT No.   06152010 Lot Specification   distribution
Medium   Ham's F12K medium with 10% FBS, 100 ug/ml heparin, 50 ug/ml endothelial cell growth supplement (ECGS). ECGS can be substituted by 10 ng/ml bFGF (FGF-2). Temperature   37 C
Cell Density at Seeding   6.6 - 8.4 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS.
Doubling Time   approx. 2 days Cell Number in Vial (cells/1ml)   6.3 x 10^5
Viability at cell freezing (%)   82.5 Antibiotics Used   free
Passage Number   P25 PDL   +10.6
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50271 Cell Name   
LOT No.   03092009 Lot Specification   distribution
Medium   Ham's F12K medium with 10% FBS, 100 ug/ml heparin, 50 ug/ml endothelial cell growth supplement (ECGS). ECGS can be substituted by 10 ng/ml bFGF (FGF-2). Temperature   37 C
Cell Density at Seeding   5 - 6 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS.
Doubling Time   46 hrs Cell Number in Vial (cells/1ml)   6.6 x 10^5 (viable)
Viability at cell freezing (%)   88.2 Antibiotics Used   free
Passage Number   P25 PDL   +13.5
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50271 Cell Name   
LOT No.   03042008 Lot Specification   distribution
Medium   Ham's F12K medium with 100 ug/ml heparin, 50 ug/ml endothelial cell growth supplment (ECGS) and 10% fetal bovine serum (GIBCO Cat#10099, lot 484375) Temperature   37 C
Cell Density at Seeding   approx. 7 x 10^4 cells/ml (1.4 x 10^4 cells/cm2) Methods for Passages   0.25% trypsin and 0.02% EDTA. Split ratio 1:4.
Doubling Time   D.T. 2 - 3 days Cell Number in Vial (cells/1ml)   6.7 x 10^5
Viability at cell freezing (%)   89 Antibiotics Used   free
Passage Number   P25 PDL   +13.5
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50271 Cell Name   
LOT No.   10312006 Lot Specification   distribution
Medium   Ham's F12K medium with 100 ug/ml heparin, 50 ug/ml endothelial cell growth supplement (Sigma) and 10% fetal bovine serum (FBS lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   5 - 10 x 10^4 cells/ml Methods for Passages   0.05-0.25% trypsin and 0.02% EDTA
Doubling Time   1.5 - 2.0 days Cell Number in Vial (cells/1ml)   8.9 x 10^5
Viability at cell freezing (%)   94.3 Antibiotics Used   free
Passage Number   P25 PDL   +14.1
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50271 Cell Name   
LOT No.   06092005 Lot Specification   distribution
Medium   Ham's F12K medium with 100 ug/ml heparin, 50 ug/ml endothelial cell growth supplement and 10% fetal bovine serum (FBS lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   1 - 2 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   30 - 40 hrs Cell Number in Vial (cells/1ml)   7.7 x 10^5
Viability at cell freezing (%)   82 Antibiotics Used   free
Passage Number   P26 PDL   13 at bank
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50271 Cell Name   
LOT No.   06252001 Lot Specification   distribution
Medium   Ham's F12K medium with 50 ug/ml ECGS (Sigma E2759), 100 ug/ml heparin and 10% FCS Temperature   37 C
Cell Density at Seeding   2-6 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. Growth at least additional 11 PDL was confirmed at bank.
Doubling Time    Cell Number in Vial (cells/1ml)   5.8 x 10^5
Viability at cell freezing (%)   77.1 Antibiotics Used   free
Passage Number   P25 PDL   12 at bank
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50271 Cell Name   HUV-EC-C
LOT No.   1127 Lot Specification   distribution
Medium   Ham's F12K with 10% FBS, 100 ug/ml heparin-Ca, and 50 ug/ml ECGS. Temperature   37 C
Cell Density at Seeding   5 x 10^4 cells/ml Methods for Passages   trypsin-EDTA
Doubling Time   4 folds/week Cell Number in Vial (cells/1ml)   4.6 x 10^5
Viability at cell freezing (%)   84.9 Antibiotics Used   free
Passage Number   P24 PDL   +12.4
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD (type B), MD, AST
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.)-culture medium CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50271 Cell Name   
LOT No.   10142003 Lot Specification   distribution
Medium   Ham's F12K medium with 10% fetal bovine serum, 50 ug/ml ECGS, 100 ug/ml heparin-Na salt (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   5-6 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   D.T. = 2 days (max) Cell Number in Vial (cells/1ml)   4.1 x 10^5
Viability at cell freezing (%)   80.3 Antibiotics Used   free
Passage Number   P25 PDL   +12.4
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture meidum CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50271 Cell Name   HUV-EC-C
LOT No.   03302015 Lot Specification   distribution
Medium   Ham's F12K medium with 10% fetal bovine serum (FBS; GIBCO Cat.# 10091), 100 ug/ml heparin and 50 ug/ml endothelial cell growth supplement Temperature   37 C
Cell Density at Seeding   2.4 - 5.1 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 1.5 day Cell Number in Vial (cells/1ml)   8.7 x 10^5
Viability at cell freezing (%)   86 Antibiotics Used   free
Passage Number   P25 PDL   +12.8
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:11,12
D13S317:9,11
D7S820:8,12
D16S539:11,12
VWA:16
TH01:6,9.3
AM:X
TPOX:8,11
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Ham's F12K CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   86
Additional information   
Cell No.   IFO50271 Cell Name   HUV-EC-C
LOT No.   10192019 Lot Specification   distribution
Medium   Ham's F12K medium with 10% FBS, 100 ug/ml heparin, 50 ug/ml endothelial cell growth supplement (ECGS). ECGS can be substituted by 10 ng/ml bFGF (FGF-2). Temperature   37 C
Cell Density at Seeding   5.3 - 7.1 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 43 hrs Cell Number in Vial (cells/1ml)   1.3 x 10^6
Viability at cell freezing (%)   87.9 Antibiotics Used   free
Passage Number   P24 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Ham's F12K CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   87.9
Additional information   
Cell No.   IFO50271 Cell Name   HUV-EC-C
LOT No.   08272018 Lot Specification   distribution
Medium   Ham's F12K medium with 10% FBS, 100 ug/ml heparin, 50 ug/ml endothelial cell growth supplement (ECGS). ECGS can be substituted by 10 ng/ml bFGF (FGF-2). Temperature   37 C
Cell Density at Seeding   3 - 7 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 2.5 days Cell Number in Vial (cells/1ml)   9.0 x 10^5
Viability at cell freezing (%)   96 Antibiotics Used   free
Passage Number   P24 PDL   +14.7
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Ham's F12K CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   90
Additional information   
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