IFO50279 NKY113

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50279 Cell Name   NKY113
Profile   SP21/0-Ag14 myeloma x spleen from mouse immunized with a human colon adenocarcinoma SW1116. This cell line produces MSW113 monoclonal antibody. MSW113 monoclonal antibody recognizes a cancer associated antigenm sialyl-Le^a oligosaccharide. Other Name   
Animal   mouse Strain   
Genus   Mus Species   musculus
Sex    Age   
Identity    Tissue for Primary Cancer   antibody-producing hybridoma
Case history   hybridoma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphoblast-like Character   MSW113 antibody, recognizing a cancer-associated olygosaccharide
Classify   hybridoma Established by   Yamashina, I. et al.
Registered by   Yamashina,I. Regulation for Distribution   
Comment    Year   1990
Medium   72% Dulbecco's MEM (high glucose), 8% NCTC109, 20% FBS, 116 mg/L arginine, 216 mg/L glutamine, 36 mg/L asparagine, 6 mg/L folic acid, 110 mg/L Na-pyruvate Methods for Passages   dilution
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   SP21/0-Ag14 x spleen
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_J276
Reference
Pubmed id:3071532Immunoaffinity isolation of a sialyl-Le(a) oligosaccharide from human milk.
Kitagawa H,Nakada H,Numata Y,Kurosaka A,Fukui S,Funakoshi I,Kawasaki T,Shimada K,Inagaki F,Yamashina I
J Biochem. 1988 Oct;104(4):591-4

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LOT Information

Cell No.   IFO50279 Cell Name   NKY113
LOT No.   556 Lot Specification   distribution
Medium   D.MEM (high glucose), 72% ; NCTC 109, 8% ; FBS, 20% ; and additive (see below *) Temperature   37 C
Cell Density at Seeding    Methods for Passages   dilution. * additive : Arg 116, Gln 216, Asn 36, folic acid 6, Na-pyruvate 110, mg/l
Doubling Time    Cell Number in Vial (cells/1ml)   3 x 10^6
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   >55 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD(type B), MD, MPI, AST
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   Lot# is labeled as R520F554.
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