IFO50280 77N1
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | IFO50280 | Cell Name | 77N1 |
|---|---|---|---|
| Profile | Parental strain is NRK (normal rat kidney) cells (J. Bacteriol.,92, 1133 (1966)). | Other Name | |
| Animal | rat | Strain | |
| Genus | Rattus | Species | norvegicus |
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | kidney | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | fibroblast-like | Character | transformant of NRK cells by avian sarcoma virus B77 |
| Classify | transformed | Established by | Hirai, R. |
| Registered by | Hirai,R. | Regulation for Distribution | |
| Comment | Year | 1990 | |
| Medium | 1:1 mixture of Ham's F12 medium and Dulbecco's modified Eagle's medium, with 5% fetal bovine serum | Methods for Passages | trypsin-EDTA |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5 % | Tissue Sampling | kidney |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Images |
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LOT Information
| Cell No. | IFO50280 | Cell Name | 77N1 |
|---|---|---|---|
| LOT No. | 841 | Lot Specification | distribution |
| Medium | Ham's F12 : D.MEM = 1:1, 95% ; FBS, 5% | Temperature | 37 C |
| Cell Density at Seeding | Methods for Passages | trypsin-EDTA | |
| Doubling Time | Cell Number in Vial (cells/1ml) | 8 x 10^5 | |
| Viability at cell freezing (%) | 96 | Antibiotics Used | free |
| Passage Number | Unknown (6 at IFO) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NP, PepB |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | 10% DMSO, 20% serum (final conc.) - culture medium | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information |