IFO50280 77N1

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50280 Cell Name   77N1
Profile   Parental strain is NRK (normal rat kidney) cells (J. Bacteriol.,92, 1133 (1966)). Other Name   
Animal   rat Strain   
Genus   Rattus Species   norvegicus
Sex    Age   
Identity    Tissue for Primary Cancer   kidney
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   transformant of NRK cells by avian sarcoma virus B77
Classify   transformed Established by   Hirai, R.
Registered by   Hirai,R. Regulation for Distribution   
Comment    Year   1990
Medium   1:1 mixture of Ham's F12 medium and Dulbecco's modified Eagle's medium, with 5% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   kidney
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_6810

LOT Information

Cell No.   IFO50280 Cell Name   77N1
LOT No.   841 Lot Specification   distribution
Medium   Ham's F12 : D.MEM = 1:1, 95% ; FBS, 5% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   8 x 10^5
Viability at cell freezing (%)   96 Antibiotics Used   free
Passage Number   Unknown (6 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, PepB
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information