IFO50280 77N1 Cell information
Cell type：general cells
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JCRB No.
IFO50280
Cell Name
77N1
Profile
Parental strain is NRK (normal rat kidney) cells (J. Bacteriol.,92, 1133 (1966)).
Other Name
Animal
rat
Strain
Genus
Rattus
Species
norvegicus
Sex
Age
Identity
Tissue for Primary Cancer
kidney
Case history
Metastasis
Tissue Metastasized
Genetics
Life Span
infinite
Crisis PDL
Morphology
fibroblast-like
Character
transformant of NRK cells by avian sarcoma virus B77
Classify
transformed
Established by
Hirai, R.
Registered by
Hirai,R.
Regulation for Distribution
Comment
Year
1990
Medium
1:1 mixture of Ham's F12 medium and Dulbecco's modified Eagle's medium, with 5% fetal bovine serum
Methods for Passages
trypsin-EDTA
Cell Number on Passage
Race
CO2 Conc.
5 %
Tissue Sampling
kidney
Tissue Type
Detection of virus genome fragment by Real-time PCR
Detected DNA Virus
not tested
Detected RNA Virus
not tested
Images
LOT Information
Cell No.
IFO50280
Cell Name
77N1
LOT No.
841
Lot Specification
distribution
Medium
Ham's F12 : D.MEM = 1:1, 95% ; FBS, 5%
Temperature
37 C
Cell Density at Seeding
Methods for Passages
trypsin-EDTA
Doubling Time
Cell Number in Vial (cells/1ml)
8 x 10^5
Viability at cell freezing (%)
96
Antibiotics Used
free
Passage Number
Unknown (6 at IFO)
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
NP, PepB
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Exoteric Gene
Medium for Freezing
10% DMSO, 20% serum (final conc.) - culture medium
CO2 Conc.
Viability immediately after thawing (%)
Additional information
