IFO50293 MA-89

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50293 Cell Name   MA-89
Profile   Not immortalized cells. Limited life span. Other Name   
Animal   mouse Strain   C3H/He
Genus   Mus Species   musclus
Sex    Age   1-3 day
Identity    Tissue for Primary Cancer   neural
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   finite Crisis PDL   
Morphology   fibroblast-like Character   normal mixed glial culture
Classify   primary Established by   Yoshida, T.
Registered by   Yoshida,T. Regulation for Distribution   
Comment    Year   1990
Medium   G5 medium (D.MEM (high glucose) with 5ug/ml insulin, 50ug/ml transferrin, 10nM hydrocortisone, 10ng/ml biotin, 30nM Na-selenite, 5ng/ml bFGF, 10ng/ml EGF) Methods for Passages   0.1% crystalline trypsin and 0.1% soybean trypsin inhibitor (Sigma type I-S)
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   brain, cerebra
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_J293
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LOT Information

Cell No.   IFO50293 Cell Name   MA-89
LOT No.   623 Lot Specification   seed/distribution
Medium   G5 medium Temperature   37 C
Cell Density at Seeding    Methods for Passages   0.1% crystalline trypsin-soybean trypsin inhibitor
Doubling Time   DT=24-36h Cell Number in Vial (cells/1ml)   4 x 10^6
Viability at cell freezing (%)   70 Antibiotics Used   free
Passage Number   P4 (from primary) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   MD, MPI, AST
Chromosome Mode   40 Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 0.1% methylcellulose - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50293 Cell Name   
LOT No.   625 Lot Specification   distribution
Medium   G5 medium Temperature   37 C
Cell Density at Seeding   (ampoule label = ASTROCYTES, 1989-9-20) Methods for Passages   0.1% crystalline trypsin-soybean trypsin inhibitor
Doubling Time   D.T.=24-36h Cell Number in Vial (cells/1ml)   3.8 x 10^6
Viability at cell freezing (%)   76 Antibiotics Used   free
Passage Number   3 (from primary) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, Pep B, LD.
Chromosome Mode   40 Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 0.1% methylcellulose - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
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