IFO50295 NB-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50295 Cell Name   NB-1
Profile   neuroblastoma Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   2
Identity   available Tissue for Primary Cancer   neural
Case history   neuroblastoma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   round-neuron-like Character   neuroblastoma, neuronal differentiation
Classify   tumor Established by   Miyake, S.
Registered by   Suzuki,T. Regulation for Distribution   
Comment    Year   1990
Medium   RPMI1640 medium with 10% fetal bovine serum Methods for Passages   0.02% EDTA-PBS
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   sympatho-adrenal cell
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1440
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 949179
Reference
Pubmed id:590688Radiosensitivity of human neuroblastoma cell line (NB-1).
Ohnuma N,Kasuga T,Nojiri I,Furuse T
Gan. 1977 Oct;68(5):711-2
Pubmed id:201151Differentiation of human neuroblastoma cells in vitro--morphological changes induced by dibutyrl cyclic AMP.
Ishikawa S
Acta Pathol Jpn. 1977 Sep;27(5):697-711
Pubmed id:12256Acetylcholine synthesis in sympathetic human neuroblastoma.
Imashuku S,Inui A,Kusunoki T,Okabe H,Yonezawa T
J Pediatr Surg. 1976 Dec;11(6):949-54
Pubmed id:174024[Morphological differentiation in vitro of human continuous and functional neuroblastoma cell line, NB-I under treatment of (But)2cAMP (author's transl)].
Miyake S,Shimo Y,Kitamura T
No Shinkei Geka. 1975 May;3(5):407-14
Pubmed id:4144593Catecholamine metabolism in tissue culture cells of a neuroblastoma.
Imashuku S,Inui A,Nakamura T,Tanaka J,Miyake S
J Clin Endocrinol Metab. 1973 May;36(5):931-6

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LOT Information

Cell No.   IFO50295 Cell Name   
LOT No.   12092005 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   0.6 - 1.4 x 10^4 cells/ml Methods for Passages   treatment with 0.02% EDTA in Ca, Mg-free PBS
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   7.1 x 10^6
Viability at cell freezing (%)   99 Antibiotics Used   free
Passage Number   Unknown (10 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:9,11
D13S317:10,13
D7S820:8,12
D16S539:9
VWA:16,19
TH01:7
AM:X
TPOX:11
CSF1PO:10,12
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50295 Cell Name   NB-1
LOT No.   980 Lot Specification   distribution
Medium   RPMI 1640, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   0.02% EDTA-PBS
Doubling Time    Cell Number in Vial (cells/1ml)   5 x 10^6
Viability at cell freezing (%)   90 Antibiotics Used   free
Passage Number   Unknown (10 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD(type B), MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50295 Cell Name   NB-1
LOT No.   09252019 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1 x 10^5 cells/mL Methods for Passages   treatment with 0.02% EDTA in Ca, Mg-free PBS
Doubling Time   approx. 32 hrs. Cell Number in Vial (cells/1ml)   3.3 x 10^6
Viability at cell freezing (%)   approx. 10 Antibiotics Used   free
Passage Number   Unknown (9 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   84.3 Additional information   
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