IFO50296 WEHI-3b

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50296 Cell Name   WEHI-3b
Profile   myelomonocyte, (BALB/c mouse) Other Name   
Animal   mouse Strain   
Genus   Mus Species   musclus
Sex    Age   
Identity    Tissue for Primary Cancer   hemo-lymphocytic
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphoblast-like Character   myelomonocyte
Classify   tumor Established by   Warner, N. L.
Registered by   Shimizu,T. Regulation for Distribution   
Comment    Year   1990
Medium   RPMI1640 medium with 5% fetal bovine serum Methods for Passages   dilution
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   myelomonocyte
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2239
Reference
Pubmed id:3012557Characterization of leukotriene A4 synthase from murine mast cells: evidence for its identity to arachidonate 5-lipoxygenase.
Shimizu T,Izumi T,Seyama Y,Tadokoro K,Rådmark O,Samuelsson B
Proc Natl Acad Sci U S A. 1986 Jun;83(12):4175-9
Pubmed id:6873525Spontaneous, in vitro, malignant transformation of a basophil/mast cell line.
Ball PE,Conroy MC,Heusser CH,Davis JM,Conscience JF
Differentiation. 1983;24(1):74-8
Pubmed id:6170720Major histocompatibility complex-restricted antigen presentation to antigen-reactive T cells by B lymphocyte tumor cells.
McKean DJ,Infante AJ,Nilson A,Kimoto M,Fathman CG,Walker E,Warner N
J Exp Med. 1981 Nov 1;154(5):1419-31
Pubmed id:894031Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS.
Ralph P,Nakoinz I
J Immunol. 1977 Sep;119(3):950-54
Pubmed id:318922Direct toxic effects of immunopotentiators on monocytic, myelomonocytic, and histiocytic or macrophage tumor cells in culture.
Ralph P,Nakoinz I
Cancer Res. 1977 Feb;37(2):546-50
Pubmed id:1083890Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines.
Ralph P,Moore MA,Nilsson K
J Exp Med. 1976 Jun 1;143(6):1528-33
Pubmed id:5259325A transplantable myelomonocytic leukemia in BALB-c mice: cytology, karyotype, and muramidase content.
Warner NL,Moore MA,Metcalf D
J Natl Cancer Inst. 1969 Oct;43(4):963-82

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LOT Information

Cell No.   IFO50296 Cell Name   
LOT No.   02032005 Lot Specification   distribution
Medium   RPMI1640 medium with 5% fetal bovine serum (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   1 - 2 x 10^5 cells/ml Methods for Passages   dilution
Doubling Time   approx. 19 hrs Cell Number in Vial (cells/1ml)   3.5 x 10^6
Viability at cell freezing (%)   86 Antibiotics Used   free
Passage Number   > P17 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50296 Cell Name   WEHI-3b
LOT No.   986 Lot Specification   distribution
Medium   RPMI 1640, 95% ; FBS, 5% ---> PRMI 1640, 97.5% ; FBS, 2.5% Temperature   37 C
Cell Density at Seeding    Methods for Passages   dilution
Doubling Time    Cell Number in Vial (cells/1ml)   3 x 10^6
Viability at cell freezing (%)   82 Antibiotics Used   free
Passage Number   Unknown (15 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD, MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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