IFO50303 T98G

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50303 Cell Name   T98G
Profile   glioblastoma, multiforme Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   61
Identity   available Tissue for Primary Cancer   neural
Case history   glioblastoma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   glioblastoma
Classify   tumor Established by   L.Hayflick, L.
Registered by   Igarashi,K. Regulation for Distribution   
Comment    Year   1990
Medium   Eagle's minimal essential medium with 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   brain
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0556
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 687586
Reference
Pubmed id:222778T98G: an anchorage-independent human tumor cell line that exhibits stationary phase G1 arrest in vitro.
Stein GH
J Cell Physiol. 1979 Apr;99(1):43-54

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LOT Information

Cell No.   IFO50303 Cell Name   T98G
LOT No.   1007 Lot Specification   distribution
Medium   E.MEM, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   1 x 10^6
Viability at cell freezing (%)   90 Antibiotics Used   free
Passage Number   Unknown (7 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD(type B), MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:10,12
D13S317:13
D7S820:9,10
D16S539:13
VWA:17,20
TH01:7,9.3
AM:X,Y
TPOX:8
CSF1PO:10,12
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50303 Cell Name   T98G
LOT No.   06152021 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   2.4 - 6.5 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 28 hrs. Cell Number in Vial (cells/1ml)   1.6 x 10^6
Viability at cell freezing (%)   99 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   99 Additional information   
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