IFO50309 SKG-II

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50309 Cell Name   SKG-II
Profile   cervical epidermoid carcinoma, large cell, non-keratinizing type Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   
Identity   available Tissue for Primary Cancer   uterine cervix
Case history   squamous carcinoma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology    Character   cervical squamous carcinoma
Classify   tumor Established by   Nozawa, S.
Registered by   Nozawa,S. Regulation for Distribution   
Comment   This cell line contains a DNA sequence for E6 oncogene of HPV type 18, but not type 16 and 33. (Tested at IFO (PCR)). Year   1991
Medium   Ham's F12 medium with 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   uterine cervix
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 +
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2794
Reference
Pubmed id:11329934 Effects of embryotrophic factors on the embryogenesis and organogenesis of mouse embryos in vitro.
Ishiwata I,Tokieda Y,Kiguchi K,Sato K,Ishikawa H
Hum Cell. 2000 Dec;13(4):185-95

Images
Movies



LOT Information

Cell No.   IFO50309 Cell Name   SKG-II
LOT No.   1001 Lot Specification   distribution
Medium   Ham's F12, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   1 x 10^6
Viability at cell freezing (%)   70 Antibiotics Used   free
Passage Number   Unknown (11 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD(type B), MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12
D13S317:8
D7S820:11
D16S539:11
VWA:16,18
TH01:7,9
AM:X
TPOX:8,9
CSF1PO:12
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   Ampule lot# is labeled as R696F1001.
Cell No.   IFO50309 Cell Name   SKG-II
LOT No.   12052018 Lot Specification   distribution
Medium   Ham's F12 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Lot # printed on ampule ”120522018” is mislabeled. Correct lot # is 12052018. Temperature   37 C
Cell Density at Seeding   1.4 - 7.4 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 47 hrs. Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)   97 Antibiotics Used   free
Passage Number   Unknown (10 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Ham's F12 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   88
Additional information   Lot# is mislabelled as 120522018 on vials. Correct lot# is 12052018.
CLOSE