Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50310 Cell Name   SKG-IIIa
Profile   cervical epidermoid carcinoma, clear cell type Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   
Identity   available Tissue for Primary Cancer   uterine cervix
Case history   uterine cervical epidermoid cancer Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology    Character   cervical epidermoid cancer
Classify   tumor Established by   Nozawa, S.
Registered by   Nozawa,S. Regulation for Distribution   
Comment   This cell line and SKG-IIIb (IFO 50311) originated from the same patient. This cell line contains a DNA sequence for E6 oncogene of HPV type 16, but not type 18 and 33. (Tested at IFO (PCR)). Year   1991
Medium   Ham's F12 medium with 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   uterine cervix
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes Integration of HPV16 to host genome (~one copy/cell) was reported (Int J Cancer 1986;37:499)

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1704
Pubmed id:6699456[Biological properties of two newly established cell lines (SKG-3a,3b) from a human uterine cervical epidermoid carcinoma].
Udagawa Y,Nozawa S,Tsukazaki K,Ohta H,Akiba T,Kurihara S
Nihon Sanka Fujinka Gakkai Zasshi. 1984 Feb;36(2):237-46
Pubmed id:6604031Sodium butyrate produces concordant expression of "early placental" alkaline phosphatase, pregnancy-specific beta 1-glycoprotein and human chronic gonadotropin beta-subunit in a newly established uterine cervical cancer cell line (SKG-IIIa).
Nozawa S,Engvall E,Kano S,Kurihara S,Fishman WH
Int J Cancer. 1983 Sep 15;32(3):267-72
Pubmed id:6600963Newly established uterine cervical cancer cell line (SKG-III) with Regan isoenzyme, human chorionic gonadotropin beta-subunit, and pregnancy-specific beta 1-glycoprotein phenotypes.
Nozawa S,Udagawa Y,Ohta H,Kurihara S,Fishman WH
Cancer Res. 1983 Apr;43(4):1748-60


LOT Information

Cell No.   IFO50310 Cell Name   SKG-IIIa
LOT No.   1004 Lot Specification   distribution
Medium   Ham's F12, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   5 x 10^6
Viability at cell freezing (%)   89 Antibiotics Used   free
Passage Number   Unknown (13 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD(type B), MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   Lot# is labeled as R697F1004 on vials