-Detailed Information [IFO50315]-

IFO50315 RMG-I

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.	IFO50315	Cell Name	RMG-I
Profile	clear cell carcinoma	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex	F	Age
Identity	available	Tissue for Primary Cancer	ovary
Case history	ovarian clear cell carcinoma	Metastasis	Yes
Tissue Metastasized	ascites	Genetics
Life Span	infinite	Crisis PDL
Morphology	polygonal or spindle	Character	clear cell carcinoma
Classify	tumor	Established by	Nozawa, S.
Registered by	Nozawa,S.	Regulation for Distribution
Comment	This cell line does not contain a DNA sequence for E6 oncogene of HPV type 16, 18 and 33. (Tested at IFO (PCR)).	Year	1991
Medium	Ham's F12 medium with 10% fetal bovine serum	Methods for Passages	trypsin-EDTA
Cell Number on Passage		Race
CO2 Conc.	5 %	Tissue Sampling	ovary
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_1662

Reference
Pubmed id:3154025	Establishment of a human ovarian clear cell carcinoma cell line (RMG-I) and its single cell cloning--with special reference to the stem cell of the tumor. Nozawa S,Tsukazaki K,Sakayori M,Jeng CH,Iizuka R Hum Cell. 1988 Dec;1(4):426-35

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LOT Information

12252012
07022008
1002
01212019
12212023

Cell No.	IFO50315	Cell Name
LOT No.	12252012	Lot Specification	distribution
Medium	Ham's F12 medium with 10% fetal bovine serum (FBS, GIBCO Cat. # 10099)	Temperature	37 C
Cell Density at Seeding	0.9 - 1.4 x 10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 72 hrs.	Cell Number in Vial (cells/1ml)	2.6 x 10^6
Viability at cell freezing (%)	67.5	Antibiotics Used	free
Passage Number	Unknown (3 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - Ham's F12	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	IFO50315	Cell Name
LOT No.	07022008	Lot Specification	distribution
Medium	Ham's F12 medium with 10% fetal bovine serum (GIBCO Cat.# 10099, lot 484375)	Temperature	37 C
Cell Density at Seeding	2 x 10^5 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS.
Doubling Time	approx. 3 days	Cell Number in Vial (cells/1ml)	1.7 x 10^6 (viable)
Viability at cell freezing (%)	52.4	Antibiotics Used	free
Passage Number	Unknown (13 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - F12	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	IFO50315	Cell Name	RMG-I
LOT No.	1002	Lot Specification	distribution
Medium	Ham's F12, 90% ; FBS, 10%	Temperature	37 C
Cell Density at Seeding		Methods for Passages	trypsin-EDTA
Doubling Time		Cell Number in Vial (cells/1ml)	5 x 10^6
Viability at cell freezing (%)	78	Antibiotics Used	free
Passage Number	Unknown (13 at IFO)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NP, G6PD(type B), MD
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing	10% DMSO, 20% serum (final conc.) - culture medium	CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	IFO50315	Cell Name	RMG-I
LOT No.	01212019	Lot Specification	distribution
Medium	Ham's F12 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012)	Temperature	37 C
Cell Density at Seeding	1.0 - 1.3 x 10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 57 hrs.	Cell Number in Vial (cells/1ml)	3.7 x 10^6
Viability at cell freezing (%)	91	Antibiotics Used	free
Passage Number	Unknown (13 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing		CO2 Conc.	5%
Viability immediately after thawing (%)	91	Additional information

Cell No.	IFO50315	Cell Name	RMG-I
LOT No.	12212023	Lot Specification	distribution
Medium	Ham's F-12 medium with 10% fetal bovine serum (FBS; NICHIREI Cat. # 17412, Lot 20M00K)	Temperature	37 C
Cell Density at Seeding	approx. 7 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 67 hrs.	Cell Number in Vial (cells/1ml)	2.4 x 10^6
Viability at cell freezing (%)	97.8	Antibiotics Used	free
Passage Number	Unknown (13 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:12 D13S317:8,12 D7S820:11 D16S539:9,10 VWA:17,18 TH01:6,7 AM:X TPOX:11 CSF1PO:10
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - Ham's F-12	CO2 Conc.	5%
Viability immediately after thawing (%)	89.9	Additional information