IFO50350 Hs68

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50350 Cell Name   Hs68
Profile   Normal diploid fibroblasts with limited life span. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   newborn
Identity   available Tissue for Primary Cancer   skin
Case history   normal Metastasis   
Tissue Metastasized    Genetics   The enzyme aspartoacylase level is low.
Life Span   finite Crisis PDL   
Morphology   fibroblast-like Character   skin normal diploid fibroblast
Classify   primary Established by   Naval Biosciences Laboratories, Oakland
Registered by   Naruo,K. Regulation for Distribution   
Comment    Year   1991
Medium   Dulbecco's modified Eagle's medium (high glucose) with 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   Caucasian
CO2 Conc.   5 % Tissue Sampling   foreskin
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0839
Reference
Pubmed id:8757838Intact immune defenses are required for mice to resist the ts-4 vaccine strain of Toxoplasma gondii.
Sayles PC,Johnson LL
Infect Immun. 1996 Aug;64(8):3088-92

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LOT Information

Cell No.   IFO50350 Cell Name   
LOT No.   01242005 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (high glucose, final 4.5 g/L) with 10% fetal bovine serum (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   3 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 1.2 days Cell Number in Vial (cells/1ml)   6.9 x 10^5
Viability at cell freezing (%)   94.9 Antibiotics Used   free
Passage Number   P24 PDL   Unk. +7
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50350 Cell Name   Hs68
LOT No.   1023 Lot Specification   distribution
Medium   D.MEM(high glucose), 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   5 x 10^5
Viability at cell freezing (%)   86 Antibiotics Used   free
Passage Number   P25 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD(type B), MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50350 Cell Name   Hs68
LOT No.   01222018 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (high glucose) with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   5.9 -9.7 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 2 days Cell Number in Vial (cells/1ml)   2.2 x 10^6
Viability at cell freezing (%)   96.0 Antibiotics Used   free
Passage Number   P25 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   96.0 Additional information   
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