IFO50354 HT-1080

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50354 Cell Name   HT-1080
Profile   fibrosarcoma, acetabulum Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   35
Identity   available Tissue for Primary Cancer   sarcoma
Case history   fibrosarcoma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   acetabulum, fibrosarcoma
Classify   tumor Established by   Rasheed, S.
Registered by   Nakamura,T. Regulation for Distribution   
Comment    Year   1991
Medium   Eagle's minimal essential medium with non-essential amino acids and 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   acetabulum
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0317
Reference
Pubmed id:2125632 Synergistic antitumor effect of glycosylated recombinant human lymphotoxin with human interferon-gamma on lymphotoxin-sensitive human tumor.
Kawatsu M,Funahashi I,Kajikawa T,Takeo K,Asahi T,Yamashita T,Kawaharada H,Watanabe K
J Interferon Res. 1990 Oct;10(5):519-29
Pubmed id:841332 Enzyme polymorphisms as genetic signatures in human cell cultures.
O'Brien SU,Kleiner G,Olson R,Shannon JE
Science. 1977 Mar 25;195(4284):1345-8
Pubmed id:4132053 Characterization of a newly derived human sarcoma cell line (HT-1080).
Rasheed S,Nelson-Rees WA,Toth EM,Arnstein P,Gardner MB
Cancer. 1974 Apr;33(4):1027-33

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LOT Information

Cell No.   IFO50354 Cell Name   HT-1080
LOT No.   1129 Lot Specification   distribution
Medium   E.MEM with NEAA, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding   1-2 x 10^5 cells/ml Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   2.4 x 10^6
Viability at cell freezing (%)   91 Antibiotics Used   free
Passage Number   Unknown (5 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:11,13
D13S317:12,14
D7S820:9,10
D16S539:9,12
VWA:14,19
TH01:6
AM:X,Y
TPOX:8
CSF1PO:12
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.)-culture medium CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   IFO50354 Cell Name   HT-1080
LOT No.   09122016 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids and 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   4.6 - 6.0 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 18 hrs. Cell Number in Vial (cells/1ml)   2.3 x 10^6
Viability at cell freezing (%)   94.7 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM with NEAA CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   94.7
Additional information   
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