-Detailed Information [IFO50356]-

IFO50356 KNS-42

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.	IFO50356	Cell Name	KNS-42
Profile	glioma, GFAP-positive, S-100 and NSE-negative	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex	M	Age	16
Identity	available	Tissue for Primary Cancer	neural
Case history	glioma	Metastasis
Tissue Metastasized		Genetics
Life Span	infinite	Crisis PDL
Morphology	polygonal	Character	glioma, GFAP-positive, S-100 and NSE-negative
Classify	tumor	Established by	Takeshita, I.
Registered by	Takeshita,I.	Regulation for Distribution
Comment		Year	1991
Medium	Eagle's minimal essential medium with 5% fetal bovine serum	Methods for Passages	trypsin-EDTA
Cell Number on Passage		Race
CO2 Conc.	5 %	Tissue Sampling	brain
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_0378

Reference
Pubmed id:2448680	Characteristics of an established human glioma cell line, KNS-42. Takeshita I,Takaki T,Kuramitsu M,Nagasaka S,Machi T,Ogawa H,Egami H,Mannoji H,Fukui M,Kitamura K Neurol Med Chir (Tokyo). 1987 Jul;27(7):581-7
Pubmed id:none	Established cell lines derived from human gliomas. Takeshita I., Takaki T., Nakamura T., Maeyama R., Fukui M., Kitamura K. Human Cell, 1990, 3:255-6

Images

LOT Information

07232010
994
05202016
03262019
06132022

Cell No.	IFO50356	Cell Name
LOT No.	07232010	Lot Specification	distribution
Medium	Eagle's minimum essential medium with 5% fetal bovine serum (GIBCO Cat. # 10099)	Temperature	37 C
Cell Density at Seeding	1.7 - 2.0 x 10^5 cells/mL	Methods for Passages	0.25% trypsin and 0.02% EDTA
Doubling Time	approx. 14 hrs	Cell Number in Vial (cells/1ml)	1.6 x 10^6
Viability at cell freezing (%)	91.2	Antibiotics Used	free
Passage Number	Unknown (7 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - EMEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	IFO50356	Cell Name	KNS-42
LOT No.	994	Lot Specification	distribution
Medium	E.MEM, 95% ; FBS, 5%	Temperature	37 C
Cell Density at Seeding		Methods for Passages	trypsin-EDTA
Doubling Time		Cell Number in Vial (cells/1ml)	7 x 10^5
Viability at cell freezing (%)	90	Antibiotics Used	free
Passage Number	Unknown (7 at IFO)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NP, G6PD(type B), MD
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing	10% DMSO, 20% serum (final conc.) - culture medium	CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	IFO50356	Cell Name	KNS-42
LOT No.	05202016	Lot Specification	distribution
Medium	Eagle's minimum essential medium with 5% fetal bovine serum (FBS; GIBCO Cat. # 10091)	Temperature	37 C
Cell Density at Seeding	3.2 - 7.0 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Passage twice a week.
Doubling Time	approx. 27 hrs	Cell Number in Vial (cells/1ml)	2.7 x 10^6
Viability at cell freezing (%)	94	Antibiotics Used	free
Passage Number	Unknown (8 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	10% DMSO, 20% FBS - EMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	94	Additional information

Cell No.	IFO50356	Cell Name	KNS-42
LOT No.	03262019	Lot Specification	distribution
Medium	Eagle's minimum essential medium with 5% fetal bovine serum (FBS; Sigma Cat. # 172012)	Temperature	37 C
Cell Density at Seeding	0.4 - 1.1 x 10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 57 hrs.	Cell Number in Vial (cells/1ml)	1.4 x 10^6
Viability at cell freezing (%)	96.0	Antibiotics Used	free
Passage Number	Unknown (7 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - EMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	94.2	Additional information

Cell No.	IFO50356	Cell Name	KNS-42
LOT No.	06132022	Lot Specification	distribution
Medium	Eagle's minimum essential medium with 5% fetal bovine serum (FBS; GIBCO Cat. # 10437)	Temperature	37 C
Cell Density at Seeding	0.6 - 1.4 x 10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approc. 42 hrs.	Cell Number in Vial (cells/1ml)	2.2 x 10^6
Viability at cell freezing (%)	approx. 100	Antibiotics Used	free
Passage Number	Unknown (7 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:12 D13S317:8 D7S820:8,11 D16S539:9,12 VWA:18,19 TH01:6 AM:X TPOX:8,11 CSF1PO:10
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - EMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	94	Additional information