IFO50356 KNS-42
Cell information
Cell type:general cells (View Pricing Information)
JCRB No. | IFO50356 | Cell Name | KNS-42 |
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Profile | glioma, GFAP-positive, S-100 and NSE-negative | Other Name | |
Animal | human | Strain | |
Genus | Homo | Species | sapiens |
Sex | M | Age | 16 |
Identity | available | Tissue for Primary Cancer | neural |
Case history | glioma | Metastasis | |
Tissue Metastasized | Genetics | ||
Life Span | infinite | Crisis PDL | |
Morphology | polygonal | Character | glioma, GFAP-positive, S-100 and NSE-negative |
Classify | tumor | Established by | Takeshita, I. |
Registered by | Takeshita,I. | Regulation for Distribution | |
Comment | Year | 1991 | |
Medium | Eagle's minimal essential medium with 5% fetal bovine serum | Methods for Passages | trypsin-EDTA |
Cell Number on Passage | Race | ||
CO2 Conc. | 5 % | Tissue Sampling | brain |
Tissue Type |
Detection of virus genome fragment by Real-time PCR | |||||||||
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Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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BKV | - | HIV-1 | - | ||||||
JCV | - | HIV-2 | - | ||||||
ADV | - | HPV18 | - | ||||||
Notes |
Reference | |
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Pubmed id:2448680 | Characteristics of an established human glioma cell line, KNS-42. Takeshita I,Takaki T,Kuramitsu M,Nagasaka S,Machi T,Ogawa H,Egami H,Mannoji H,Fukui M,Kitamura K Neurol Med Chir (Tokyo). 1987 Jul;27(7):581-7 |
Pubmed id:none | Established cell lines derived from human gliomas. Takeshita I., Takaki T., Nakamura T., Maeyama R., Fukui M., Kitamura K. Human Cell, 1990, 3:255-6 |
Images |
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LOT Information
Cell No. | IFO50356 | Cell Name | |
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LOT No. | 07232010 | Lot Specification | distribution |
Medium | Eagle's minimum essential medium with 5% fetal bovine serum (GIBCO Cat. # 10099) | Temperature | 37 C |
Cell Density at Seeding | 1.7 - 2.0 x 10^5 cells/mL | Methods for Passages | 0.25% trypsin and 0.02% EDTA |
Doubling Time | approx. 14 hrs | Cell Number in Vial (cells/1ml) | 1.6 x 10^6 |
Viability at cell freezing (%) | 91.2 | Antibiotics Used | free |
Passage Number | Unknown (7 at bank) | PDL | |
Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD. |
Chromosome Mode | Chromosome Information | ||
Surface Antigen | DNA Profile (STR) | ||
Adhesion | Yes | Exoteric Gene | |
Medium for Freezing | 10% DMSO, 20% FBS - EMEM | CO2 Conc. | 5% |
Viability immediately after thawing (%) | Additional information |
Cell No. | IFO50356 | Cell Name | KNS-42 |
---|---|---|---|
LOT No. | 994 | Lot Specification | distribution |
Medium | E.MEM, 95% ; FBS, 5% | Temperature | 37 C |
Cell Density at Seeding | Methods for Passages | trypsin-EDTA | |
Doubling Time | Cell Number in Vial (cells/1ml) | 7 x 10^5 | |
Viability at cell freezing (%) | 90 | Antibiotics Used | free |
Passage Number | Unknown (7 at IFO) | PDL | |
Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
Sterility: FUNGI | - | Isozyme Analysis | NP, G6PD(type B), MD |
Chromosome Mode | Chromosome Information | ||
Surface Antigen | DNA Profile (STR) | ||
Adhesion | Exoteric Gene | ||
Medium for Freezing | 10% DMSO, 20% serum (final conc.) - culture medium | CO2 Conc. | |
Viability immediately after thawing (%) | Additional information |
Cell No. | IFO50356 | Cell Name | KNS-42 |
---|---|---|---|
LOT No. | 05202016 | Lot Specification | distribution |
Medium | Eagle's minimum essential medium with 5% fetal bovine serum (FBS; GIBCO Cat. # 10091) | Temperature | 37 C |
Cell Density at Seeding | 3.2 - 7.0 x 10^4 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Passage twice a week. |
Doubling Time | approx. 27 hrs | Cell Number in Vial (cells/1ml) | 2.7 x 10^6 |
Viability at cell freezing (%) | 94 | Antibiotics Used | free |
Passage Number | Unknown (8 at bank) | PDL | |
Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
Sterility: FUNGI | - | Isozyme Analysis | NT |
Chromosome Mode | NT | Chromosome Information | NT |
Surface Antigen | NT | DNA Profile (STR) | |
Adhesion | Yes | Exoteric Gene | NT |
Medium for Freezing | 10% DMSO, 20% FBS - EMEM | CO2 Conc. | 5% |
Viability immediately after thawing (%) | 94 | Additional information |
Cell No. | IFO50356 | Cell Name | KNS-42 |
---|---|---|---|
LOT No. | 03262019 | Lot Specification | distribution |
Medium | Eagle's minimum essential medium with 5% fetal bovine serum (FBS; Sigma Cat. # 172012) | Temperature | 37 C |
Cell Density at Seeding | 0.4 - 1.1 x 10^5 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
Doubling Time | approx. 57 hrs. | Cell Number in Vial (cells/1ml) | 1.4 x 10^6 |
Viability at cell freezing (%) | 96.0 | Antibiotics Used | free |
Passage Number | Unknown (7 at bank) | PDL | |
Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
Sterility: FUNGI | - | Isozyme Analysis | |
Chromosome Mode | Chromosome Information | ||
Surface Antigen | DNA Profile (STR) | ||
Adhesion | Yes | Exoteric Gene | |
Medium for Freezing | 10% DMSO, 20% FBS - EMEM | CO2 Conc. | 5% |
Viability immediately after thawing (%) | 94.2 | Additional information |
Cell No. | IFO50356 | Cell Name | KNS-42 |
---|---|---|---|
LOT No. | 06132022 | Lot Specification | distribution |
Medium | Eagle's minimum essential medium with 5% fetal bovine serum (FBS; GIBCO Cat. # 10437) | Temperature | 37 C |
Cell Density at Seeding | 0.6 - 1.4 x 10^5 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
Doubling Time | approc. 42 hrs. | Cell Number in Vial (cells/1ml) | 2.2 x 10^6 |
Viability at cell freezing (%) | approx. 100 | Antibiotics Used | free |
Passage Number | Unknown (7 at bank) | PDL | |
Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
Sterility: FUNGI | - | Isozyme Analysis | |
Chromosome Mode | Chromosome Information | ||
Surface Antigen | DNA Profile (STR) | D5S818:12 D13S317:8 D7S820:8,11 D16S539:9,12 VWA:18,19 TH01:6 AM:X TPOX:8,11 CSF1PO:10 |
|
Adhesion | Yes | Exoteric Gene | |
Medium for Freezing | 10% DMSO, 20% FBS - EMEM | CO2 Conc. | 5% |
Viability immediately after thawing (%) | 94 | Additional information |