IFO50356 KNS-42

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50356 Cell Name   KNS-42
Profile   glioma, GFAP-positive, S-100 and NSE-negative Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   16
Identity   available Tissue for Primary Cancer   neural
Case history   glioma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   polygonal Character   glioma, GFAP-positive, S-100 and NSE-negative
Classify   tumor Established by   Takeshita, I.
Registered by   Takeshita,I. Regulation for Distribution   
Comment    Year   1991
Medium   Eagle's minimal essential medium with 5% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   brain
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0378
Reference
Pubmed id:2448680Characteristics of an established human glioma cell line, KNS-42.
Takeshita I,Takaki T,Kuramitsu M,Nagasaka S,Machi T,Ogawa H,Egami H,Mannoji H,Fukui M,Kitamura K
Neurol Med Chir (Tokyo). 1987 Jul;27(7):581-7
Pubmed id:noneEstablished cell lines derived from human gliomas.
Takeshita I., Takaki T., Nakamura T., Maeyama R., Fukui M., Kitamura K.
Human Cell, 1990, 3:255-6

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LOT Information

Cell No.   IFO50356 Cell Name   
LOT No.   07232010 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 5% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   1.7 - 2.0 x 10^5 cells/mL Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 14 hrs Cell Number in Vial (cells/1ml)   1.6 x 10^6
Viability at cell freezing (%)   91.2 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50356 Cell Name   KNS-42
LOT No.   994 Lot Specification   distribution
Medium   E.MEM, 95% ; FBS, 5% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   7 x 10^5
Viability at cell freezing (%)   90 Antibiotics Used   free
Passage Number   Unknown (7 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD(type B), MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50356 Cell Name   KNS-42
LOT No.   05202016 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 5% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   3.2 - 7.0 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Passage twice a week.
Doubling Time   approx. 27 hrs Cell Number in Vial (cells/1ml)   2.7 x 10^6
Viability at cell freezing (%)   94 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   94 Additional information   
Cell No.   IFO50356 Cell Name   KNS-42
LOT No.   03262019 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 5% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   0.4 - 1.1 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 57 hrs. Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   96.0 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   94.2 Additional information   
Cell No.   IFO50356 Cell Name   KNS-42
LOT No.   06132022 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 5% fetal bovine serum (FBS; GIBCO Cat. # 10437) Temperature   37 C
Cell Density at Seeding   0.6 - 1.4 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approc. 42 hrs. Cell Number in Vial (cells/1ml)   2.2 x 10^6
Viability at cell freezing (%)   approx. 100 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12
D13S317:8
D7S820:8,11
D16S539:9,12
VWA:18,19
TH01:6
AM:X
TPOX:8,11
CSF1PO:10
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   94 Additional information   
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