-Detailed Information [IFO50358]-

IFO50358 KNS-62

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.	IFO50358	Cell Name	KNS-62
Profile	bronchial squamous carcinoma, metastasis to brain	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex	M	Age	49
Identity	available	Tissue for Primary Cancer	lung (cancer)
Case history	bronchial squamous cancer	Metastasis	Yes
Tissue Metastasized	brain	Genetics
Life Span	infinite	Crisis PDL
Morphology	epithelial-like	Character	bronchial squamous carcinoma, metastasis to brain
Classify	tumor	Established by	Takaki, T.
Registered by	Takeshita,I.	Regulation for Distribution
Comment		Year	1991
Medium	Eagle's minimal essential medium with 20% fetal bovine serum	Methods for Passages	trypsin-EDTA
Cell Number on Passage		Race
CO2 Conc.	5 %	Tissue Sampling	lung
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_1335

Reference
Pubmed id:7358770	An epithelial cell line (KNS-62) derived from a brain metastasis of bronchial squamous cell carcinoma. Takaki T J Cancer Res Clin Oncol. 1980 Jan;96(1):27-33

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LOT Information

1031
1013
08282014
07122018
07202023

Cell No.	IFO50358	Cell Name	KNS-62
LOT No.	1031	Lot Specification	distribution
Medium	E.MEM, 80% ; FBS, 20%	Temperature	37 C
Cell Density at Seeding		Methods for Passages	trypsin-EDTA
Doubling Time		Cell Number in Vial (cells/1ml)	3 x 10^6
Viability at cell freezing (%)	59	Antibiotics Used	free
Passage Number	Unknown (7 at IFO)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NP, G6PD(type B), MD
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing	10% DMSO, 20% serum (final conc.) - culture medium	CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	IFO50358	Cell Name	KNS-62
LOT No.	1013	Lot Specification	distribution
Medium	E.MEM, 80% ; FBS, 20%	Temperature	37 C
Cell Density at Seeding		Methods for Passages	trypsin-EDTA
Doubling Time		Cell Number in Vial (cells/1ml)	2 x 10^6
Viability at cell freezing (%)	46	Antibiotics Used	free
Passage Number	Unknown (8 at IFO)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NP, G6PD(type B), MD
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing	10% DMSO, 20% serum (final conc.) - culture medium	CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	IFO50358	Cell Name	KNS-62
LOT No.	08282014	Lot Specification	distribution
Medium	Eagle's minimal essential medium with 20% fetal bovine serum (FBS; GIBCO Cat. # 10091)	Temperature	37 C
Cell Density at Seeding	6.4 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA
Doubling Time		Cell Number in Vial (cells/1ml)	3.2 x 10^6
Viability at cell freezing (%)	91	Antibiotics Used	free
Passage Number	Unknown (7 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:9 D13S317:11 D7S820:11 D16S539:9,11 VWA:17 TH01:7,9 AM:X TPOX:8 CSF1PO:9,12
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - EMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	91	Additional information

Cell No.	IFO50358	Cell Name	KNS-62
LOT No.	07122018	Lot Specification	distribution
Medium	Eagle's minimum essential medium with 20% fetal bovine serum (FBS; Signa Cat. # 172012)	Temperature	37 C
Cell Density at Seeding	0.9 - 1.6 x 10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 37 hrs.	Cell Number in Vial (cells/1ml)	2.4 x 10^6
Viability at cell freezing (%)	97.7	Antibiotics Used	free
Passage Number	Unknown (7 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - EMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	84.4	Additional information

Cell No.	IFO50358	Cell Name	KNS-62
LOT No.	07202023	Lot Specification	distribution
Medium	Eagle's minimum essential medium with 20% fetal bovine serum (FBS; GIBCO Cat. # 10437, Lot 1072322).	Temperature	37 C
Cell Density at Seeding	1.0 - 3.6 x 10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 33 hrs.	Cell Number in Vial (cells/1ml)	3.3 x 10^6
Viability at cell freezing (%)	98	Antibiotics Used	free
Passage Number	Unknown (7 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:9 D13S317:11 D7S820:11 D16S539:9,11 VWA:17 TH01:7,9 AM:X TPOX:8 CSF1PO:9,12
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - EMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	91	Additional information	The trypsin treatment may require 20 min. when the cells firmly attach to the dishes.