IFO50361 B2-17

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50361 Cell Name   B2-17
Profile   astrocytoma, thymidine kinase-deficient mutant of U-251 MG cells Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   75
Identity   available Tissue for Primary Cancer   neural
Case history   astrocytoma Metastasis   
Tissue Metastasized    Genetics   thymidine kinase-deficient
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   astrocytoma, thymidine kinase-deficient mutant of U-251 MG cells, GFAP-negative
Classify   mutant Established by   Takeshita, I.
Registered by   Takeshita,I. Regulation for Distribution   
Comment   The DNA fingerprint profile confirmed that this cell line derives from U-251 MG cell line. Year   1991
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum and 100ug/ml bromodeoxyuridine Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   brain
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2864
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LOT Information

Cell No.   IFO50361 Cell Name   B2-17
LOT No.   1017 Lot Specification   distribution
Medium   D.MEM, 90% ; FBS, 10% ; bromodeoxyuridine, 100 ug/ml Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   8 x 10^5
Viability at cell freezing (%)   91 Antibiotics Used   free
Passage Number   Unknown (8 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD(type B), MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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