IFO50364 L6

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50364 Cell Name   L6
Profile   skeletal muscle myoblast Other Name   
Animal   rat Strain   
Genus   Rattus Species   norvegicus
Sex    Age   
Identity    Tissue for Primary Cancer   muscular system
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   skeletal muscle myoblast
Classify    Established by   Yaffe, D. et al.
Registered by   Sasada,R. Regulation for Distribution   
Comment    Year   1991
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   skeletal myoblast
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0385
Reference
Pubmed id:2533272Spectinomycin operon of Micrococcus luteus: evolutionary implications of organization and novel codon usage.
Ohama T,Muto A,Osawa S
J Mol Evol. 1989 Nov;29(5):381-95
Pubmed id:6336315A human T cell-specific cDNA clone encodes a protein having extensive homology to immunoglobulin chains.
Yanagi Y,Yoshikai Y,Leggett K,Clark SP,Aleksander I,Mak TW
Nature. 1984 Mar 8-14;308(5955):145-9
Pubmed id:5481965The in vitro cultivation and differentiation capacities of myogenic cell lines.
Richler C,Yaffe D
Dev Biol. 1970 Sep;23(1):1-22
Pubmed id:5245982Retention of differentiation potentialities during prolonged cultivation of myogenic cells.
Yaffe D
Proc Natl Acad Sci U S A. 1968 Oct;61(2):477-83




LOT Information

Cell No.   IFO50364 Cell Name   
LOT No.   01262010 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   5.4 - 7.3 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. Passage twice a week.
Doubling Time   approx. 22 hrs Cell Number in Vial (cells/1ml)   3.1 x 10^6
Viability at cell freezing (%)   91.0 Antibiotics Used   free
Passage Number   P28 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as rat by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50364 Cell Name   L6
LOT No.   1128 Lot Specification   distribution
Medium   D.MEM, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding   1-2 x 10^5 cells/ml Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   3.6 x 10^6
Viability at cell freezing (%)   83 Antibiotics Used   free
Passage Number   P27 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD, PepB
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.)-culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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