IFO50409 NCTC clone 929
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | IFO50409 | Cell Name | NCTC clone 929 |
|---|---|---|---|
| Profile | A clone of L cells, and often referred as L929. The L cells was established from normal subcutaneous areolar and adipose tissue. | Other Name | |
| Animal | mouse | Strain | C3H/An |
| Genus | Mus | Species | musclus |
| Sex | M | Age | |
| Identity | Tissue for Primary Cancer | skin | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | fibroblast-like | Character | fibroblast, subclone of strain L |
| Classify | Established by | Sanford, K. K. | |
| Registered by | Takeuchi,M. | Regulation for Distribution | |
| Comment | Year | 1993 | |
| Medium | Eagle's minimal essential medium with non-essential amino acids and 10% fetal bovine serum | Methods for Passages | trypsin-EDTA |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5 % | Tissue Sampling | connective tissue |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:178611 | Establishment and characterization of a human histiocytic lymphoma cell line (U-937). Sundström C,Nilsson K Int J Cancer. 1976 May 15;17(5):565-77 |
| Pubmed id:13655076 | Rapid proliferation of sublines of NCTC clone 929 (strain L) mouse cells in a simple chemically defined medium (MB 752/1). WAYMOUTH C J Natl Cancer Inst. 1959 May;22(5):1003-17 |
| Pubmed id:13271674 | The minimum vitamin requirements of the L and HeLa cells in tissue culture, the production of specific vitamin deficiencies, and their cure. EAGLE H J Exp Med. 1955 Nov 1;102(5):595-600 |
| Pubmed id:13035407 | A quantitative study of the effect of certain chemically defined media on the proliferation in vitro of strain L cells from the mouse. EVANS VJ,SHANNON JE Jr,BRYANT JC,WALTZ HK,EARLE WR,SANFORD KK J Natl Cancer Inst. 1953 Feb;13(4):773-84 |
LOT Information
| Cell No. | IFO50409 | Cell Name | |
|---|---|---|---|
| LOT No. | 04262010 | Lot Specification | distribution |
| Medium | Eagle's minimum essential medium with non-essential amino acids (0.1 mM each) and 10% fetal bovine serum (GIBCO Cat. # 10099) | Temperature | 37 C |
| Cell Density at Seeding | approx. 4 - 5 x 10^4 cells/ml | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | approx. 20 - 25 hrs | Cell Number in Vial (cells/1ml) | 1.9 x 10^6 |
| Viability at cell freezing (%) | 97.3 | Antibiotics Used | free |
| Passage Number | Unknown (11 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as mouse by NP, G6PD, MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - culture medium | CO2 Conc. | 5% |
| RFLP | Viability immediately after thawing (%) | ||
| Additional information |
| Cell No. | IFO50409 | Cell Name | |
|---|---|---|---|
| LOT No. | 04042008 | Lot Specification | distribution |
| Medium | Eagle's minimum essential medium with non-essential amino acids and 10% fetal bovine serum | Temperature | 37 C |
| Cell Density at Seeding | approx. 5 x 10^4 cells/ml | Methods for Passages | 0.25% trypsin and 0.02% EDTA |
| Doubling Time | approx. 30 hrs | Cell Number in Vial (cells/1ml) | 1.9 x 10^6 |
| Viability at cell freezing (%) | 95.5 | Antibiotics Used | free |
| Passage Number | P11* | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as mouse by NP, G6PD, MD |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - culture medium | CO2 Conc. | 5% |
| RFLP | Viability immediately after thawing (%) | ||
| Additional information |
| Cell No. | IFO50409 | Cell Name | NCTC clone 929 |
|---|---|---|---|
| LOT No. | 1075 | Lot Specification | distribution |
| Medium | E.MEM with NEAA, 90% ; FBS, 10% | Temperature | 37 C |
| Cell Density at Seeding | Methods for Passages | trypsin--EDTA | |
| Doubling Time | Cell Number in Vial (cells/1ml) | 10 x 10^5 | |
| Viability at cell freezing (%) | 92 | Antibiotics Used | free |
| Passage Number | Unknown (7 at IFO) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NP, G6PD, AST |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | 10% DMSO, 20% serum (final conc.) - culture medium | CO2 Conc. | |
| RFLP | Viability immediately after thawing (%) | ||
| Additional information |
| Cell No. | IFO50409 | Cell Name | NCTC clone 929 |
|---|---|---|---|
| LOT No. | 08042016 | Lot Specification | distribution |
| Medium | Eagle's minimum essential medium with non-essential amino acids and 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Cell name of ampoule label is incorrect. Correctly NCTC clone 929. | Temperature | 37 C |
| Cell Density at Seeding | 4.2 - 6.5 x 10^4 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | approx. 23 hrs. | Cell Number in Vial (cells/1ml) | 2.2 x 10^6 |
| Viability at cell freezing (%) | 88 | Antibiotics Used | free |
| Passage Number | Unknown (7 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - culture medium | CO2 Conc. | 5% |
| RFLP | Viability immediately after thawing (%) | 88 | |
| Additional information | Mislabeled as NTCT clone 929 on vials. Correct name is NCTC clone 929. |