IFO50414 CHO-K1
Cell information
Important Notice(s)CHO-K1はプロリン要求性です。必ず指定培地をご利用ください。
Cell type：general cells (View Pricing Information)
| JCRB No. | IFO50414 | Cell Name | CHO-K1 |
|---|---|---|---|
| Profile | CHO-K1 cells are useful for isolation of nutritionally deficient mutants. | Other Name | |
| Animal | hamster, Chinese | Strain | Chinese |
| Genus | Cricetulus | Species | griseus |
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | ovary | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | epitherial like | Character | widely used for production of recombinant proteins |
| Classify | Established by | Puck, T. T. | |
| Registered by | Takeuchi,M. | Regulation for Distribution | |
| Comment | Year | 1993 | |
| Medium | Ham's F12 medium with 10% fetal bovine serum | Methods for Passages | trypsin-EDTA |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5 % | Tissue Sampling | ovary |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | not tested | ||||||
| CMV | - | parvoB19 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| EBV | - | HBV | - | ||||||
| HHV6 | - | HTLV-1 | - | ||||||
| HHV7 | - | HTLV-2 | - | ||||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
LOT Information
| Cell No. | IFO50414 | Cell Name | |
|---|---|---|---|
| LOT No. | 07072008 | Lot Specification | distribution |
| Medium | Ham's F12 medium with 10% fetal bovine serum (GIBCO Cat.# 10099, lot 484375) | Temperature | 37 C |
| Cell Density at Seeding | inoculum 10^5 cells/ml -> confluent after 2 days. | Methods for Passages | 0.25% trypsin and 0.02% EDTA |
| Doubling Time | 17 hrs | Cell Number in Vial (cells/1ml) | 3.0 x 10^6 |
| Viability at cell freezing (%) | 98.7 | Antibiotics Used | free |
| Passage Number | Unknown (8 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as Chinese hamster by NP, G6PD, MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - F12 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | IFO50414 | Cell Name | |
|---|---|---|---|
| LOT No. | 06022003 | Lot Specification | distribution |
| Medium | Ham's F12 medium with 10% fetal bovine serum (FBS lot; BioWhittaker 0S069F) | Temperature | 37 C |
| Cell Density at Seeding | 5 x 10^4 cells/ml | Methods for Passages | |
| Doubling Time | D.T. = 13 - 23 hrs | Cell Number in Vial (cells/1ml) | 3.2 x 10^6 |
| Viability at cell freezing (%) | 97 | Antibiotics Used | free |
| Passage Number | Unknown(7 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as Chinese hamster by NP, G6PD, AST. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS-Ham's F12 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | IFO50414 | Cell Name | CHO-K1 |
|---|---|---|---|
| LOT No. | 1132 | Lot Specification | distribution |
| Medium | Ham's F12, 90% ; FBS, 10% | Temperature | 37 C |
| Cell Density at Seeding | 5-10 x 10^3 cells/ml | Methods for Passages | trypsin-EDTA |
| Doubling Time | Cell Number in Vial (cells/1ml) | 1.8 x 10^6 | |
| Viability at cell freezing (%) | 89.0 | Antibiotics Used | free |
| Passage Number | Unknown (5 at IFO) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NP, G6PD, PepB |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | 10% DMSO, 20% serum (final conc.)-culture medium | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | IFO50414 | Cell Name | CHO-K1 |
|---|---|---|---|
| LOT No. | 06272013 | Lot Specification | distribution |
| Medium | Ham's F12 medium with 10% fetal bovine serum (FBS: GIBCO Cat. No. 10099) | Temperature | 37C |
| Cell Density at Seeding | 0.5-1.5 10^5 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA. |
| Doubling Time | Cell Number in Vial (cells/1ml) | 5.8 x 10^6 cells/1mL | |
| Viability at cell freezing (%) | Antibiotics Used | free | |
| Passage Number | Unkown (9 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | G6PD,NP,LDH were examined. Chinese hamster confirmed. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | Culture medium with 10% DMSO | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 98.3 | Additional information |