IFO50418 ASF-4-1

Cell information

Important Notice(s)

本細胞株の分譲に際しての寄託者の承諾について (On the distribution of this cell line. An approval of depositor is required.)

ASF-4シリーズ線維芽細胞の使用目的の限定について / The limitation of use for ASF-4 series fibroblasts

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50418 Cell Name   ASF-4-1
Profile   Normal diploid (46, XY) fibroblast with limited life span. The ASF-4-1 and the ASF-4-2 lines were established from skins of the same donor at different ages. This cell line was taken from the donor at 36 years and 2 month-old Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   36.2
Identity   available Tissue for Primary Cancer   skin
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   finite Crisis PDL   66.5
Morphology    Character   skin normal diploid fibroblast
Classify   primary Established by   Kaji, K.
Registered by   Kaji,K. Regulation for Distribution   Limited to the research for scenescence / rejuvenation. Developer's approval is required.
Comment    Year   1993
Medium   Eagle's minimal essential medium with 10% fetal bovine serum Methods for Passages   trypsin
Cell Number on Passage   ca 1 x 10^4 cells/cm2, weekly Race   Japanese
CO2 Conc.   5 % Tissue Sampling   skin fibroblast from upper arm
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2854
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LOT Information

Cell No.   IFO50418 Cell Name   
LOT No.   07222002 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum (lot; BioWhittaker 0S069F) Temperature   37 C
Cell Density at Seeding   3-6 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA.
Doubling Time   D.T. = 2-2.5 days Cell Number in Vial (cells/1ml)   8.7 x 10^5
Viability at cell freezing (%)   89.6 Antibiotics Used   free
Passage Number   P6 at bank PDL   34
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS-EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50418 Cell Name   ASF-4-1
LOT No.   847 Lot Specification   seed/distribution
Medium   E.MEM, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   5 x 10^5
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   Unknown (2 at IFO) PDL   25
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   not tested
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:9,10
D13S317:11,12
D7S820:10,11
D16S539:10,11
VWA:17,18
TH01:8,9
AM:XY
TPOX:11
CSF1PO:10,13
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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Cell No.   IFO50418 Cell Name   ASF-4-1
LOT No.   11212017 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sgma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   approx. 2 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 41 hrs. Cell Number in Vial (cells/1ml)   6.1 x 10^5
Viability at cell freezing (%)   94 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   PDL=34
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   94 Additional information   
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