IFO50422 PKF
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | IFO50422 | Cell Name | PKF |
|---|---|---|---|
| Profile | The name PKF derives from Pig Kidney Fibroblastic. | Other Name | |
| Animal | pig | Strain | |
| Genus | Sus | Species | scrofa |
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | kidney | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | fibroblast-like | Character | susceptible to Vaccinia virus |
| Classify | Established by | Ito, H. | |
| Registered by | Ito,H. | Regulation for Distribution | |
| Comment | Year | 1993 | |
| Medium | Eagle's minimal essential medium with 3% fetal bovine serum and 0.3% lactoalbumin hydrolysate | Methods for Passages | trypsin-EDTA |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5 % | Tissue Sampling | kidney |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
LOT Information
| Cell No. | IFO50422 | Cell Name | PKF |
|---|---|---|---|
| LOT No. | 1024 | Lot Specification | distribution |
| Medium | E.MEM with 3%FBS, 0.3% lactoalbumin hydrolysate | Temperature | 37 C |
| Cell Density at Seeding | Methods for Passages | trypsin-EDTA | |
| Doubling Time | Cell Number in Vial (cells/1ml) | 1 x 10^6 | |
| Viability at cell freezing (%) | 86 | Antibiotics Used | free |
| Passage Number | Unknown (6 at IFO) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NP, LD |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | 10% DMSO, 20% serum (final conc.) - culture medium | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information |